CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010389
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Pyruvate kinase 
Protein Synonyms/Alias
 PK 
Gene Name
 pyk 
Gene Synonyms/Alias
 MPN_303; MP533 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
107ETAPEGLKILKDSEVacetylation[1]
184NDHTIFTKKRLNLPNacetylation[1]
202SIPFLSAKDLRDIDFacetylation[1]
249KHVKLIAKIESNHALacetylation[1]
294YWQRYMIKACRFFNKacetylation[1]
475SLSKAEFKSLANVAIacetylation[1]
504CLGVFHNKIISL***acetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
  
Sequence Annotation
 METAL 58 58 Potassium (By similarity).
 METAL 60 60 Potassium (By similarity).
 METAL 90 90 Potassium (By similarity).
 METAL 91 91 Potassium; via carbonyl oxygen (By
 METAL 251 251 Magnesium (By similarity).
 METAL 275 275 Magnesium (By similarity).
 BINDING 56 56 Substrate (By similarity).
 BINDING 274 274 Substrate; via amide nitrogen (By
 BINDING 275 275 Substrate; via amide nitrogen (By
 BINDING 307 307 Substrate (By similarity).  
Keyword
 Allosteric enzyme; ATP-binding; Complete proteome; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Potassium; Pyruvate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MIHHLKRTKI IATCGPALTK KLWTLAMLDD PAYAAMKAEA YANIENIIKN GVTVIRLNFS 60
HGNHEEQAVR IKIVRDVAKK LNLPVSIMLD TNGPEIRVFE TAPEGLKILK DSEVVINTTT 120
KEVAKNNQFS VSDASGTYNM VNDVKVGQKI LVDDGKLSLV VKRIDTKNNQ VICVAQNDHT 180
IFTKKRLNLP NADYSIPFLS AKDLRDIDFG LTHQIDYIAA SFVNTTENIK QLRDYLASKN 240
AKHVKLIAKI ESNHALNNID GIIKASDGIM VARGDLGLEI PYYKVPYWQR YMIKACRFFN 300
KRVITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETANGAFP LNAVYVMKMI 360
DKQSETFFDY QYNLNYYMAN SKARHSEFWK QVVLPLAQKT APKRKLINSD FKYDFVVHAT 420
NNLNEIYALS NARLAAAVII LTNDPQVYTG HGVDYGIFPY LIDQKPQSLS KAEFKSLANV 480
AIKHYQQHGE ISQLKQCLGV FHNKIISL 508 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015806; Pyrv_Knase_insert_dom. 
Pfam
 PF00224; PK 
SMART
  
PROSITE
 PS00110; PYRUVATE_KINASE 
PRINTS
 PR01050; PYRUVTKNASE.