CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucosidase 2 subunit beta 
Protein Synonyms/Alias
 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH 
Gene Name
 Prkcsh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
142REGFRLKKILIEEWKacetylation[1]
142REGFRLKKILIEEWKsuccinylation[1]
149KILIEEWKTAREEKQacetylation[2]
166LLELQAGKKSLEDQVacetylation[1]
166LLELQAGKKSLEDQVsuccinylation[1]
452WAGPDHDKFSAMKYEacetylation[2]
457HDKFSAMKYEQGTGCacetylation[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Regulatory subunit of glucosidase II. 
Sequence Annotation
 DOMAIN 209 244 EF-hand 1.
 DOMAIN 245 290 EF-hand 2.
 DOMAIN 406 461 PRKCSH.
 MOTIF 518 521 Prevents secretion from ER (Potential).
 MOD_RES 89 89 Phosphoserine; by PKC (Potential).
 MOD_RES 376 376 Phosphoserine; by PKC (Potential).
 MOD_RES 383 383 Phosphoserine; by PKC (Potential).
 MOD_RES 427 427 Phosphoserine; by PKC (Potential).
 CARBOHYD 72 72 N-linked (GlcNAc...) (Potential).
 CARBOHYD 469 469 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Calcium; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 521 AA 
Protein Sequence
MLLLLLLLLP LCWAVEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ VNDDYCDCKD 60
GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC DGTDEYNSGT VCENTCREKG 120
RKEKESLQQL AEVTREGFRL KKILIEEWKT AREEKQSKLL ELQAGKKSLE DQVETLRAAK 180
EEAERPEKEA KDQHRKLWEE QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL 240
DTDGDGALSE EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE 300
KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP PYDEETQAII 360
DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE FAYLYSQCYE LTTNEYVYRL 420
CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK 480
ETVVTSTTEP SRCEYLMELM TPAACPEPPP EAPSDGDHDE L 521 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IDA:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0001889; P:liver development; IGI:MGI.
 GO:0006491; P:N-glycan processing; TAS:MGI.
 GO:0010977; P:negative regulation of neuron projection development; IMP:MGI.
 GO:0006807; P:nitrogen compound metabolic process; IGI:MGI.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0072001; P:renal system development; IGI:MGI. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR026874; Glucosidase_2_bsu.
 IPR002172; LDrepeatLR_classA_rpt.
 IPR009011; Man6P_isomerase_rcpt-bd_dom.
 IPR012913; PRKCSH. 
Pfam
 PF13202; EF_hand_3
 PF07915; PRKCSH 
SMART
 SM00192; LDLa 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00014; ER_TARGET 
PRINTS