UniProt Accession

 SRP54_YEAST; P20424; D6W489 

Genbank Protein ID

 X16908; M55517; X51614; U51033; BK006949 

Genbank Nucleotide ID

 CAA34781.1; AAA35092.1; CAA35952.1; AAB68136.1; DAA11505.1 

Protein Name

 Signal recognition particle subunit SRP54 

Protein Synonyms/Alias

 Signal recognition particle 54 kDa protein homolog 

Gene Name

 SRP54 

Gene Synonyms/Alias

 SRH1; YPR088C; P9513.14 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
100	TCEGSEEKAFVPKKR	ubiquitination	[1]
186	VAEEGINKFKKEKFD	acetylation	[2]
335	MENIQKGKFTLLDFK	acetylation	[2]
342	KFTLLDFKKQMQTIM	acetylation	[2]
489	MMQQAQQKLKQNPGL	ubiquitination	[1]
536	PQMQQMAKQFGMG**	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPAse- activating proteins (GAPs), thereby resolving their association. 

Sequence Annotation

 NP_BIND 116 123 GTP (By similarity).
 NP_BIND 198 202 GTP (By similarity).
 NP_BIND 256 259 GTP (By similarity).
 REGION 1 303 G-domain.
 REGION 304 541 M-domain.  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; GTPase activation; Nucleotide-binding; Reference proteome; Ribonucleoprotein; RNA-binding; Signal recognition particle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 541 AA 

Protein Sequence

Gene Ontology

 GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IDA:SGD.
 GO:0008312; F:7S RNA binding; IDA:SGD.
 GO:0005525; F:GTP binding; ISA:SGD.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005048; F:signal sequence binding; IPI:SGD.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0006617; P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; ISA:SGD. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR027417; P-loop_NTPase.
 IPR013822; Signal_recog_particl_SRP54_hlx.
 IPR004125; Signal_recog_particle_SRP54_M.
 IPR022941; SRP54.
 IPR006325; SRP54_euk.
 IPR000897; SRP54_GTPase_dom. 

Pfam

 PF00448; SRP54
 PF02881; SRP54_N
 PF02978; SRP_SPB 

SMART

 SM00382; AAA
 SM00962; SRP54
 SM00963; SRP54_N 

PROSITE

 PS00300; SRP54 

PRINTS