CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017120
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium uptake protein 2, mitochondrial 
Protein Synonyms/Alias
 EF-hand domain-containing family member A1 
Gene Name
 MICU2 
Gene Synonyms/Alias
 EFHA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82HGIIYIGKPSLRKQRubiquitination[1, 2, 3, 4, 5, 6]
194DGNEMIEKREFFKLQubiquitination[2, 4, 5]
199IEKREFFKLQKIISKubiquitination[4]
206KLQKIISKQDDLMTVubiquitination[1, 2, 4, 6]
214QDDLMTVKTNETGYQubiquitination[1, 2, 6]
226GYQEAIVKEPEINTTubiquitination[2, 4, 5]
355AEFKRAVKVATGQELubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in mitochondrial uniporter-mediated calcium uptake. Probable regulator of the mitochondrial calcium uniporter (MCU) (By similarity). 
Sequence Annotation
 DOMAIN 172 207 EF-hand 1.
 DOMAIN 227 262 EF-hand 2.
 DOMAIN 293 328 EF-hand 3.
 DOMAIN 362 397 EF-hand 4.
 MOD_RES 205 205 Phosphoserine.  
Keyword
 Complete proteome; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 434 AA 
Protein Sequence
MAAAAGSCAR VAAWGGKLRR GLAVSRQAVR SPGPLAAAVA GAALAGAGAA WHHSRVSVAA 60
RDGSFTVSAQ KNVEHGIIYI GKPSLRKQRF MQFSSLEHEG EYYMTPRDFL FSVMFEQMER 120
KTSVKKLTKK DIEDTLSGIQ TAGCGSTFFR DLGDKGLISY TEYLFLLTIL TKPHSGFHVA 180
FKMLDTDGNE MIEKREFFKL QKIISKQDDL MTVKTNETGY QEAIVKEPEI NTTLQMRFFG 240
KRGQRKLHYK EFRRFMENLQ TEIQEMEFLQ FSKGLSFMRK EDFAEWLLFF TNTENKDIYW 300
KNVREKLSAG ESISLDEFKS FCHFTTHLED FAIAMQMFSL AHRPVRLAEF KRAVKVATGQ 360
ELSNNILDTV FKIFDLDGDE CLSHEEFLGV LKNRMHRGLW VPQHQSIQEY WKCVKKESIK 420
GVKEVWKQAG KGLF 434 
Gene Ontology
 GO:0034704; C:calcium channel complex; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0051561; P:elevation of mitochondrial calcium ion concentration; ISS:UniProtKB.
 GO:0006851; P:mitochondrial calcium ion transport; ISS:UniProtKB. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 
Pfam
  
SMART
 SM00054; EFh 
PROSITE
 PS50222; EF_HAND_2 
PRINTS