UniProt Accession

 1433E_MOUSE; P62259; P29360; P42655; Q63631 

Genbank Protein ID

 Z19599; D87663; AF483478; AF483479; BC058686 

Genbank Nucleotide ID

 CAA79659.1; BAA13424.1; AAL90752.1; AAL90753.1; AAH58686.1 

Protein Name

 14-3-3 protein epsilon 

Protein Synonyms/Alias

 14-3-3E 

Gene Name

 Ywhae 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
12	EDLVYQAKLAEQAER	ubiquitination	[1]
29	EMVESMKKVAGMDVE	ubiquitination	[1]
50	NLLSVAYKNVIGARR	ubiquitination	[1]
69	IISSIEQKEENKGGE	ubiquitination	[1]
73	IEQKEENKGGEDKLK	ubiquitination	[1]
78	ENKGGEDKLKMIREY	acetylation	[2]
78	ENKGGEDKLKMIREY	ubiquitination	[1]
106	DILDVLDKHLIPAAN	ubiquitination	[1]
118	AANTGESKVFYYKMK	ubiquitination	[1]
123	ESKVFYYKMKGDYHR	ubiquitination	[1]
125	KVFYYKMKGDYHRYL	ubiquitination	[1]
142	FATGNDRKEAAENSL	acetylation	[3]
142	FATGNDRKEAAENSL	ubiquitination	[1]
196	DRACRLAKAAFDDAI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 50 50 N6-acetyllysine (By similarity).
 MOD_RES 69 69 N6-acetyllysine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 123 123 N6-acetyllysine (By similarity).
 MOD_RES 210 210 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005871; C:kinesin complex; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0019904; F:protein domain specific binding; IDA:MGI.
 GO:0021987; P:cerebral cortex development; IMP:MGI.
 GO:0021766; P:hippocampus development; IMP:MGI.
 GO:0035308; P:negative regulation of protein dephosphorylation; IDA:MGI.
 GO:0001764; P:neuron migration; IMP:MGI.
 GO:0006605; P:protein targeting; IDA:MGI. 

Interpro

 IPR000308; 14-3-3.
 IPR023409; 14-3-3_CS.
 IPR023410; 14-3-3_domain. 

Pfam

 PF00244; 14-3-3 

SMART

 SM00101; 14_3_3 

PROSITE

 PS00796; 1433_1
 PS00797; 1433_2 

PRINTS

 PR00305; 1433ZETA.