CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002971
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucosamine-6-phosphate deaminase 
Protein Synonyms/Alias
 GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase 
Gene Name
 nagB 
Gene Synonyms/Alias
 glmD; b0678; JW0664 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
14TTAEQVGKWAARHIVacetylation[1]
28VNRINAFKPTADRPFacetylation[1]
57KALVEMHKAGQVSFKacetylation[1]
79DEYVGLPKEHPESYYacetylation[1]
124ECRQYEEKIRSYGKIacetylation[1]
130EKIRSYGKIHLFMGGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion. 
Sequence Annotation
 ACT_SITE 72 72 Proton acceptor; for enolization step.
 ACT_SITE 141 141 For ring-opening step.
 ACT_SITE 143 143 Proton acceptor; for ring-opening step.
 ACT_SITE 148 148 For ring-opening step.
 DISULFID 219 219 Interchain.  
Keyword
 3D-structure; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Disulfide bond; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 266 AA 
Protein Sequence
MRLIPLTTAE QVGKWAARHI VNRINAFKPT ADRPFVLGLP TGGTPMTTYK ALVEMHKAGQ 60
VSFKHVVTFN MDEYVGLPKE HPESYYSFMH RNFFDHVDIP AENINLLNGN APDIDAECRQ 120
YEEKIRSYGK IHLFMGGVGN DGHIAFNEPA SSLASRTRIK TLTHDTRVAN SRFFDNDVNQ 180
VPKYALTVGV GTLLDAEEVM ILVLGSQKAL ALQAAVEGCV NHMWTISCLQ LHPKAIMVCD 240
EPSTMELKVK TLRYFNELEA ENIKGL 266 
Gene Ontology
 GO:0004342; F:glucosamine-6-phosphate deaminase activity; IDA:EcoCyc.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0006046; P:N-acetylglucosamine catabolic process; IMP:EcoCyc.
 GO:0019262; P:N-acetylneuraminate catabolic process; IEA:UniProtKB-UniPathway.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IGI:EcoCyc. 
Interpro
 IPR006148; Glc/Gal-6P_isomerase.
 IPR004547; Glucosamine6P_isomerase.
 IPR018321; Glucosamine6P_isomerase_CS. 
Pfam
 PF01182; Glucosamine_iso 
SMART
  
PROSITE
 PS01161; GLC_GALNAC_ISOMERASE 
PRINTS