CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000013
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synaptosomal-associated protein 23 
Protein Synonyms/Alias
 SNAP-23; Vesicle-membrane fusion protein SNAP-23 
Gene Name
 SNAP23 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40ESQDAGIKTITMLDEubiquitination[1]
49ITMLDEQKEQLNRIEubiquitination[1, 2, 3, 4]
64EGLDQINKDMRETEKubiquitination[1, 3, 4]
97TKNFESGKAYKTTWGubiquitination[4]
141AASGGYIKRITNDARubiquitination[1, 4]
185DAQNPQIKRITDKADubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. 
Sequence Annotation
 DOMAIN 14 76 t-SNARE coiled-coil homology 1.
 DOMAIN 146 208 t-SNARE coiled-coil homology 2.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 20 20 Phosphoserine (By similarity).
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 110 110 Phosphoserine.
 LIPID 79 79 S-palmitoyl cysteine.
 LIPID 80 80 S-palmitoyl cysteine.
 LIPID 83 83 S-palmitoyl cysteine.
 LIPID 85 85 S-palmitoyl cysteine.
 LIPID 87 87 S-palmitoyl cysteine.
 LIPID 112 112 S-palmitoyl cysteine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell junction; Cell membrane; Coiled coil; Complete proteome; Direct protein sequencing; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Protein transport; Reference proteome; Repeat; Synapse; Synaptosome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 211 AA 
Protein Sequence
MDNLSSEEIQ QRAHQITDES LESTRRILGL AIESQDAGIK TITMLDEQKE QLNRIEEGLD 60
QINKDMRETE KTLTELNKCC GLCVCPCNRT KNFESGKAYK TTWGDGGENS PCNVVSKQPG 120
PVTNGQLQQP TTGAASGGYI KRITNDARED EMEENLTQVG SILGNLKDMA LNIGNEIDAQ 180
NPQIKRITDK ADTNRDRIDI ANARAKKLID S 211 
Gene Ontology
 GO:0042582; C:azurophil granule; IDA:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0042581; C:specific granule; IDA:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-KW.
 GO:0006944; P:cellular membrane fusion; TAS:ProtInc.
 GO:0006887; P:exocytosis; IEA:Compara.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006903; P:vesicle targeting; TAS:ProtInc. 
Interpro
 IPR000928; SNAP-25.
 IPR000727; T_SNARE_dom. 
Pfam
 PF00835; SNAP-25
 PF05739; SNARE 
SMART
 SM00397; t_SNARE 
PROSITE
 PS50192; T_SNARE 
PRINTS