UniProt Accession

 PYRD_ECOLI; P0A7E1; P05021 

Genbank Protein ID

 X02826; U00096; AP009048 

Genbank Nucleotide ID

 CAA26594.1; AAC74031.1; BAA35700.1 

Protein Name

 Dihydroorotate dehydrogenase (quinone) 

Protein Synonyms/Alias

 DHOdehase; DHOD; DHODase; Dihydroorotate oxidase 

Gene Name

 pyrD 

Gene Synonyms/Alias

 b0945; JW0928 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
128	DNLVENVKKAHYDGV	acetylation	[1]
144	GINIGKNKDTPVEQG	acetylation	[1]
152	DTPVEQGKDDYLICM	acetylation	[1]
210	DLQAMHHKYVPIAVK	acetylation	[1]
258	RSLVQGMKNCDQTGG	acetylation	[1]
330	FKGPPLIKEIVTHI*	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. 

Sequence Annotation

 NP_BIND 62 66 FMN.
 NP_BIND 318 319 FMN.
 REGION 111 115 Substrate binding.
 REGION 246 247 Substrate binding.
 ACT_SITE 175 175 Nucleophile.
 BINDING 66 66 Substrate.
 BINDING 86 86 FMN; via amide nitrogen.
 BINDING 139 139 FMN.
 BINDING 172 172 FMN.
 BINDING 172 172 Substrate.
 BINDING 177 177 Substrate.
 BINDING 217 217 FMN.
 BINDING 245 245 FMN; via carbonyl oxygen.
 BINDING 268 268 FMN; via amide nitrogen.
 BINDING 297 297 FMN; via amide nitrogen.  

Keyword

 3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; Membrane; Oxidoreductase; Pyrimidine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 336 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IDA:EcoCyc.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0004152; F:dihydroorotate dehydrogenase activity; IDA:EcoCyc.
 GO:0004158; F:dihydroorotate oxidase activity; IEA:InterPro.
 GO:0010181; F:FMN binding; IDA:EcoCyc.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IMP:EcoCyc.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR012135; Dihydroorotate_DH_1_2.
 IPR005719; Dihydroorotate_DH_2.
 IPR001295; Dihydroorotate_DH_CS. 

Pfam

 PF01180; DHO_dh 

SMART

  

PROSITE

 PS00911; DHODEHASE_1
 PS00912; DHODEHASE_2 

PRINTS