CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009235
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Octanoyltransferase 
Protein Synonyms/Alias
 Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase 
Gene Name
 lipB 
Gene Synonyms/Alias
 b0630; JW5089 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
92MYVLLNLKRRKLGVRacetylation[1]
134PGVYVGEKKICSLGLacetylation[1]
183MAKISQWKPEATTNNacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate. 
Sequence Annotation
 REGION 71 78 Substrate binding (By similarity).
 REGION 138 140 Substrate binding (By similarity).
 REGION 151 153 Substrate binding (By similarity).
 ACT_SITE 169 169 Acyl-thioester intermediate.  
Keyword
 Acyltransferase; Complete proteome; Cytoplasm; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 213 AA 
Protein Sequence
MYQDKILVRQ LGLQPYEPIS QAMHEFTDTR DDSTLDEIWL VEHYPVFTQG QAGKAEHILM 60
PGDIPVIQSD RGGQVTYHGP GQQVMYVLLN LKRRKLGVRE LVTLLEQTVV NTLAELGIEA 120
HPRADAPGVY VGEKKICSLG LRIRRGCSFH GLALNVNMDL SPFLRINPCG YAGMEMAKIS 180
QWKPEATTNN IAPRLLENIL ALLNNPDFEY ITA 213 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0033819; F:lipoyl(octanoyl) transferase activity; IEA:EC.
 GO:0017118; F:lipoyltransferase activity; IDA:EcoliWiki.
 GO:0016415; F:octanoyltransferase activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IDA:EcoliWiki.
 GO:0009107; P:lipoate biosynthetic process; IMP:EcoliWiki.
 GO:0010629; P:negative regulation of gene expression; IDA:EcoliWiki. 
Interpro
 IPR004143; BPL_LipA_LipB.
 IPR000544; Octanoyltransferase.
 IPR020605; Octanoyltransferase_CS. 
Pfam
 PF03099; BPL_LplA_LipB 
SMART
  
PROSITE
 PS01313; LIPB 
PRINTS