CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009965
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial 
Protein Synonyms/Alias
 Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma 
Gene Name
 Idh3g 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
149YANVIHCKSLPGVVTacetylation[1]
159PGVVTRHKDIDILIVacetylation[1, 2]
206RIAEYAFKLAQESGRacetylation[2, 3]
226VHKANIMKLGDGLFLacetylation[1, 2, 4]
226VHKANIMKLGDGLFLsuccinylation[4]
Reference
 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
  
Sequence Annotation
 NP_BIND 56 84 NAD (Potential).
 NP_BIND 309 316 ATP (Potential).
 METAL 254 254 Magnesium or manganese (By similarity).
 BINDING 136 136 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 254 254 Substrate (By similarity).
 MOD_RES 363 363 Phosphothreonine.  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Nucleotide-binding; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 393 AA 
Protein Sequence
MALKVAIAAG GAAKAMLKPT LLCRPWEVLA AHVAPRRSIS SQQTIPPSAK YGGRHTVTMI 60
PGDGIGPELM LHVKSVFRHA CVPVDFEEVH VSSNADEEDI RNAIMAIRRN RVALKGNIET 120
NHNLPPSHKS RNNILRTSLD LYANVIHCKS LPGVVTRHKD IDILIVRENT EGEYSSLEHE 180
SVAGVVESLK IITKAKSLRI AEYAFKLAQE SGRKKVTAVH KANIMKLGDG LFLQCCREVA 240
AHYPQITFDS MIVDNTTMQL VSRPQQFDVM VMPNLYGNIV NNVCAGLVGG PGLVAGANYG 300
HVYAVFETAT RNTGKSIANK NIANPTATLL ASCMMLDHLK LHSYATSIRK AVLASMDNEN 360
MHTPDIGGQG TTSQAIQDII RHIRIINGRA VEA 393 
Gene Ontology
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR004434; Isocitrate_DH_NAD.
 IPR024084; IsoPropMal-DH-like_dom. 
Pfam
 PF00180; Iso_dh 
SMART
  
PROSITE
 PS00470; IDH_IMDH 
PRINTS