UniProt Accession

 NFIL3_HUMAN; Q16649; B2R9Y8; Q14211; Q6FGQ8; Q96HS0 

Genbank Protein ID

 U26173; X64318; S79880; EF028070; CR542049; AK313970; AL353764; CH471089; BC008197 

Genbank Nucleotide ID

 AAA93067.1; CAA45597.1; AAB35410.1; ABK15691.1; CAG46846.1; BAG36685.1; CAH73854.1; EAW62796.1; AAH08197.1 

Protein Name

 Nuclear factor interleukin-3-regulated protein 

Protein Synonyms/Alias

 E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A 

Gene Name

 NFIL3 

Gene Synonyms/Alias

 E4BP4; IL3BP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
350	NEFEATQKLSSPIDM	ubiquitination	[1]
360	SPIDMTSKRHFELEK	ubiquitination	[1, 2]
406	HQKELSGKTQNSFKT	ubiquitination	[1]
412	GKTQNSFKTGVVEMK	ubiquitination	[1, 2]
419	KTGVVEMKDSGYKVS	ubiquitination	[1, 3]
424	EMKDSGYKVSDPENL	ubiquitination	[1, 2, 3]
434	DPENLYLKQGIANLS	ubiquitination	[1]
448	SAEVVSLKRLIATQP	ubiquitination	[2]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B- site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity). 

Sequence Annotation

 DOMAIN 73 136 bZIP.
 REGION 79 95 Basic motif (By similarity).
 REGION 99 106 Leucine-zipper (By similarity).
 REGION 299 363 Necessary for transcriptional repression
 MOD_RES 286 286 Phosphoserine (By similarity).
 MOD_RES 301 301 Phosphoserine.
 MOD_RES 353 353 Phosphoserine.  

Keyword

 Activator; Biological rhythms; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 462 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:HPA.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:BHF-UCL.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IEA:Compara.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0007623; P:circadian rhythm; IEA:InterPro.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara. 

Interpro

 IPR004827; bZIP.
 IPR016743; TF_bZIP_E4BP4.
 IPR010533; Vert_IL3-reg_TF. 

Pfam

 PF07716; bZIP_2
 PF06529; Vert_IL3-reg_TF 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS