CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005758
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-hydroxyisobutyrate dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 HIBADH 
Gene Name
 Hibadh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
55NMGNPMAKNLIKHGYacetylation[1]
78PDVCKEFKEAGEQVAacetylation[1]
94SPADVAEKADRIITMacetylation[1]
120SGANGILKKVKKGSLacetylation[1]
140TIDPSVSKELAKEVEacetylation[1]
144SVSKELAKEVEKMGAacetylation[1]
148ELAKEVEKMGAVFMDacetylation[1]
237IRSGLDPKLLAKILNacetylation[1]
241LDPKLLAKILNMSSGacetylation[1]
296QDSATSTKTPILLGSacetylation[1]
319MCSKGYSKKDFSSVFacetylation[1]
320CSKGYSKKDFSSVFQacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 NP_BIND 39 68 NAD (By similarity).
 NP_BIND 102 103 NAD (By similarity).
 ACT_SITE 208 208 Probable.
 BINDING 107 107 NAD (By similarity).
 BINDING 133 133 NAD; via amide nitrogen (By similarity).
 BINDING 283 283 NAD (By similarity).  
Keyword
 Branched-chain amino acid catabolism; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 335 AA 
Protein Sequence
MAASLGFRGA ASGLRYWSGR RRPVGSLAAV CSRSMASKTP VGFIGLGNMG NPMAKNLIKH 60
GYPLILYDVF PDVCKEFKEA GEQVASSPAD VAEKADRIIT MLPSSMNSIE VYSGANGILK 120
KVKKGSLLID SSTIDPSVSK ELAKEVEKMG AVFMDAPVSG GVGAARSGNL TFMVGGVENE 180
FAAAQELLGC MGSNVLYCGA VGSGQSAKIC NNMLLAISMI GTAEAMNLGI RSGLDPKLLA 240
KILNMSSGRC WSSDTYNPVP GVMDGVPSSN NYQGGFGTTL MAKDLGLAQD SATSTKTPIL 300
LGSVAHQIYR MMCSKGYSKK DFSSVFQYLR EEETF 335 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; ISS:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity; IEA:InterPro.
 GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
 GO:0006574; P:valine catabolic process; ISS:UniProtKB. 
Interpro
 IPR002204; 3-OH-isobutyrate_DH-rel_CS.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR006115; 6PGDH_NADP-bd.
 IPR013328; DH_multihelical.
 IPR015815; HIBADH-type.
 IPR011548; IsoBut3OH_DH.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF03446; NAD_binding_2 
SMART
  
PROSITE
 PS00895; 3_HYDROXYISOBUT_DH 
PRINTS