UniProt Accession

 S29A1_HUMAN; Q99808; B3KQY5; Q5T9W9; Q9UJY2 

Genbank Protein ID

 U81375; AF190884; AF079117; AF495730; AK090615; AL139392; BC001382; BC008954 

Genbank Nucleotide ID

 AAC51103.1; AAF02777.1; AAC62495.1; AAM11785.1; BAG52197.1; CAI20093.1; AAH01382.1; AAH08954.1 

Protein Name

 Equilibrative nucleoside transporter 1 

Protein Synonyms/Alias

 Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside transporter, es-type; Solute carrier family 29 member 1 

Gene Name

 SLC29A1 

Gene Synonyms/Alias

 ENT1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
239	YRYYQQLKLEGPGEQ	ubiquitination	[1, 2, 3, 4]
249	GPGEQETKLDLISKG	ubiquitination	[1, 2, 3, 4, 5]
255	TKLDLISKGEEPRAG	ubiquitination	[1, 2, 3, 4]
263	GEEPRAGKEESGVSV	ubiquitination	[3, 4]
283	TNESHSIKAILKNIS	ubiquitination	[3]
287	HSIKAILKNISVLAF	ubiquitination	[3]
356	AVFMWPGKDSRWLPS	ubiquitination	[1, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

 Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). 

Sequence Annotation

 CARBOHYD 48 48 N-linked (GlcNAc...).  

Keyword

 Cell membrane; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 456 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
 GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005337; F:nucleoside transmembrane transporter activity; TAS:ProtInc.
 GO:0071333; P:cellular response to glucose stimulus; IEA:Compara.
 GO:0071456; P:cellular response to hypoxia; IEA:Compara.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
 GO:0015858; P:nucleoside transport; IDA:UniProtKB.
 GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IEA:Compara.
 GO:0030431; P:sleep; IEA:Compara.
 GO:0015862; P:uridine transport; IEA:Compara. 

Interpro

 IPR002259; Eqnu_transpt. 

Pfam

 PF01733; Nucleoside_tran 

SMART

  

PROSITE

  

PRINTS

 PR01130; DERENTRNSPRT.