UniProt Accession

 ASGL1_HUMAN; Q7L266; B2R7Q0; Q567Q4; Q6P1P0; Q8NI34; Q9H6F7 

Genbank Protein ID

 AF411076; AK025969; AK091894; AK313069; CH471076; BC021295; BC064963; BC093070 

Genbank Nucleotide ID

 AAM28434.1; BAB15302.1; BAG52437.1; BAG35897.1; EAW74012.1; AAH21295.3; AAH64963.1; AAH93070.1 

Protein Name

 Isoaspartyl peptidase/L-asparaginase 

Protein Synonyms/Alias

 Asparaginase-like protein 1; Beta-aspartyl-peptidase; Isoaspartyl dipeptidase; L-asparagine amidohydrolase; Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain 

Gene Name

 ASRGL1 

Gene Synonyms/Alias

 ALP; CRASH 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
177	GAVALDCKGNVAYAT	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L- Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine. 

Sequence Annotation

 REGION 196 199 Substrate binding.
 REGION 219 222 Substrate binding.
 ACT_SITE 168 168 Nucleophile.
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Autocatalytic cleavage; Complete proteome; Cytoplasm; Hydrolase; Protease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 308 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004067; F:asparaginase activity; IDA:UniProtKB.
 GO:0008798; F:beta-aspartyl-peptidase activity; IDA:UniProtKB.
 GO:0033345; P:asparagine catabolic process via L-aspartate; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR000246; Peptidase_T2. 

Pfam

 PF01112; Asparaginase_2 

SMART

  

PROSITE

  

PRINTS