CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004138
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cystic fibrosis transmembrane conductance regulator 
Protein Synonyms/Alias
 CFTR; ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel 
Gene Name
 CFTR 
Gene Synonyms/Alias
 ABCC7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
688ETKKQSFKQTGEFGEubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. 
Sequence Annotation
 DOMAIN 81 365 ABC transmembrane type-1 1.
 DOMAIN 423 646 ABC transporter 1.
 DOMAIN 859 1155 ABC transmembrane type-1 2.
 DOMAIN 1210 1443 ABC transporter 2.
 NP_BIND 458 465 ATP 1 (Potential).
 NP_BIND 1244 1251 ATP 2 (Potential).
 MOTIF 1478 1480 PDZ-binding.
 MOD_RES 291 291 Phosphothreonine.
 MOD_RES 549 549 Phosphoserine.
 MOD_RES 660 660 Phosphoserine; by PKA.
 MOD_RES 686 686 Phosphoserine; by PKC.
 MOD_RES 700 700 Phosphoserine; by PKA.
 MOD_RES 712 712 Phosphoserine; by PKA.
 MOD_RES 717 717 Phosphothreonine.
 MOD_RES 737 737 Phosphoserine; by PKA.
 MOD_RES 753 753 Phosphoserine; by PKA.
 MOD_RES 768 768 Phosphoserine; by PKA.
 MOD_RES 790 790 Phosphoserine; by PKC.
 MOD_RES 795 795 Phosphoserine; by PKA.
 MOD_RES 813 813 Phosphoserine; by PKA.
 MOD_RES 1444 1444 Phosphoserine.
 MOD_RES 1456 1456 Phosphoserine.
 LIPID 524 524 S-palmitoyl cysteine.
 LIPID 1395 1395 S-palmitoyl cysteine.
 CARBOHYD 894 894 N-linked (GlcNAc...).
 CARBOHYD 900 900 N-linked (GlcNAc...).
 CROSSLNK 688 688 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell membrane; Chloride; Chloride channel; Complete proteome; Disease mutation; Endosome; Glycoprotein; Hydrolase; Ion channel; Ion transport; Isopeptide bond; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1480 AA 
Protein Sequence
MQRSPLEKAS VVSKLFFSWT RPILRKGYRQ RLELSDIYQI PSVDSADNLS EKLEREWDRE 60
LASKKNPKLI NALRRCFFWR FMFYGIFLYL GEVTKAVQPL LLGRIIASYD PDNKEERSIA 120
IYLGIGLCLL FIVRTLLLHP AIFGLHHIGM QMRIAMFSLI YKKTLKLSSR VLDKISIGQL 180
VSLLSNNLNK FDEGLALAHF VWIAPLQVAL LMGLIWELLQ ASAFCGLGFL IVLALFQAGL 240
GRMMMKYRDQ RAGKISERLV ITSEMIENIQ SVKAYCWEEA MEKMIENLRQ TELKLTRKAA 300
YVRYFNSSAF FFSGFFVVFL SVLPYALIKG IILRKIFTTI SFCIVLRMAV TRQFPWAVQT 360
WYDSLGAINK IQDFLQKQEY KTLEYNLTTT EVVMENVTAF WEEGFGELFE KAKQNNNNRK 420
TSNGDDSLFF SNFSLLGTPV LKDINFKIER GQLLAVAGST GAGKTSLLMV IMGELEPSEG 480
KIKHSGRISF CSQFSWIMPG TIKENIIFGV SYDEYRYRSV IKACQLEEDI SKFAEKDNIV 540
LGEGGITLSG GQRARISLAR AVYKDADLYL LDSPFGYLDV LTEKEIFESC VCKLMANKTR 600
ILVTSKMEHL KKADKILILH EGSSYFYGTF SELQNLQPDF SSKLMGCDSF DQFSAERRNS 660
ILTETLHRFS LEGDAPVSWT ETKKQSFKQT GEFGEKRKNS ILNPINSIRK FSIVQKTPLQ 720
MNGIEEDSDE PLERRLSLVP DSEQGEAILP RISVISTGPT LQARRRQSVL NLMTHSVNQG 780
QNIHRKTTAS TRKVSLAPQA NLTELDIYSR RLSQETGLEI SEEINEEDLK ECFFDDMESI 840
PAVTTWNTYL RYITVHKSLI FVLIWCLVIF LAEVAASLVV LWLLGNTPLQ DKGNSTHSRN 900
NSYAVIITST SSYYVFYIYV GVADTLLAMG FFRGLPLVHT LITVSKILHH KMLHSVLQAP 960
MSTLNTLKAG GILNRFSKDI AILDDLLPLT IFDFIQLLLI VIGAIAVVAV LQPYIFVATV 1020
PVIVAFIMLR AYFLQTSQQL KQLESEGRSP IFTHLVTSLK GLWTLRAFGR QPYFETLFHK 1080
ALNLHTANWF LYLSTLRWFQ MRIEMIFVIF FIAVTFISIL TTGEGEGRVG IILTLAMNIM 1140
STLQWAVNSS IDVDSLMRSV SRVFKFIDMP TEGKPTKSTK PYKNGQLSKV MIIENSHVKK 1200
DDIWPSGGQM TVKDLTAKYT EGGNAILENI SFSISPGQRV GLLGRTGSGK STLLSAFLRL 1260
LNTEGEIQID GVSWDSITLQ QWRKAFGVIP QKVFIFSGTF RKNLDPYEQW SDQEIWKVAD 1320
EVGLRSVIEQ FPGKLDFVLV DGGCVLSHGH KQLMCLARSV LSKAKILLLD EPSAHLDPVT 1380
YQIIRRTLKQ AFADCTVILC EHRIEAMLEC QQFLVIEENK VRQYDSIQKL LNERSLFRQA 1440
ISPSDRVKLF PHRNSSKCKS KPQIAALKEE TEEEVQDTRL 1480 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
 GO:0016323; C:basolateral plasma membrane; NAS:UniProtKB.
 GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:Compara.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005902; C:microvillus; IEA:Compara.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0005224; F:ATP-binding and phosphorylation-dependent chloride channel activity; TAS:ProtInc.
 GO:0005260; F:channel-conductance-controlling ATPase activity; NAS:UniProtKB.
 GO:0019869; F:chloride channel inhibitor activity; IDA:UniProtKB.
 GO:0030165; F:PDZ domain binding; IDA:UniProtKB.
 GO:0015701; P:bicarbonate transport; IEA:Compara.
 GO:0032870; P:cellular response to hormone stimulus; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IEA:Compara.
 GO:0030301; P:cholesterol transport; IEA:Compara.
 GO:0015705; P:iodide transport; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0045909; P:positive regulation of vasodilation; IEA:Compara.
 GO:0007585; P:respiratory gaseous exchange; TAS:ProtInc.
 GO:0034097; P:response to cytokine stimulus; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0043627; P:response to estrogen stimulus; IEA:Compara.
 GO:0043434; P:response to peptide hormone stimulus; IEA:Compara.
 GO:0030321; P:transepithelial chloride transport; IEA:Compara.
 GO:0042311; P:vasodilation; IEA:Compara.
 GO:0006833; P:water transport; IEA:Compara. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR005291; cAMP_cl_channel.
 IPR025837; CFTR_reg_dom.
 IPR009147; CysFib_conduc_TM.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran
 PF14396; CFTR_R 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS
 PR01851; CYSFIBREGLTR.