CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014815
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 518A 
Protein Synonyms/Alias
  
Gene Name
 ZNF518A 
Gene Synonyms/Alias
 KIAA0335; ZNF518 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24LKKDYDVKNEIVDRSubiquitination[1]
77YRKLFQSKQQTARKSubiquitination[1]
286RHVITLHKEHLYAKEubiquitination[1]
416NASSGFMKTAVLGPTubiquitination[1, 2, 3]
433NVMMKNNKLAVSPNYubiquitination[1, 3, 4, 5]
464VLKLVPIKQNVCSPGubiquitination[1, 3]
524SSSILSGKASSEKEMubiquitination[3]
529SGKASSEKEMTLISQubiquitination[1]
671QRESSSSKTVVQQPIubiquitination[1]
749ECATEKSKWEDFSNVubiquitination[1]
807DSSNTPNKGLPLHCDubiquitination[1]
902IITQQLVKDKLRATTubiquitination[4, 5]
928LLNSEQKKTIIVQTSubiquitination[3]
1075LSEQQSTKLNISDSVubiquitination[1]
1121KTELLKPKLVQNSTYubiquitination[4, 5]
1185QIIGGEQKEPESRDAubiquitination[2]
1405TTYDDFSKRHKTFKPubiquitination[1]
1438KNNYQIVKTTSENILubiquitination[4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 ZN_FING 150 172 C2H2-type 1.
 ZN_FING 207 229 C2H2-type 2.
 ZN_FING 234 256 C2H2-type 3.
 ZN_FING 262 285 C2H2-type 4.
 ZN_FING 1449 1471 C2H2-type 5.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1483 AA 
Protein Sequence
MPSEQKQLFC DEKQTTLKKD YDVKNEIVDR SAPKPKISGS IHYALKNVKI DLPKINIPNE 60
VLLKHEVDKY RKLFQSKQQT ARKSISIKTV SCVEECTLLH KSERAEEEGV KMSAKILNFS 120
CLKCRDNTRY SPNDLQKHFQ MWHHGELPSY PCEMCNFSAN DFQVFKQHRR THRSTLVKCD 180
ICNNESVYTL LNLTKHFTST HCVNGNFQCE KCKFSTQDVG TFVQHIHRHN EIHYKCGKCH 240
HVCFTKGELQ KHLHIHSGTF PFTCQYCSYG ATRREHLVRH VITLHKEHLY AKEKLEKDKY 300
EKRMAKTSAG LKLILKRYKI GASRKTFWKR KKINSGSDRS IEKNTQVLKK MNKTQTKSED 360
QSHVVQEHLS EEKDERLHCE NNDKAPESES EKPTPLSTGQ GNRAEEGPNA SSGFMKTAVL 420
GPTLKNVMMK NNKLAVSPNY NATFMGFKMM DGKQHIVLKL VPIKQNVCSP GSQSGAAKDG 480
TANLQPQTLD TNGFLTGVTT ELNDTVYMKA ATPFSCSSSI LSGKASSEKE MTLISQRNNM 540
LQTMDYEKSV SSLSATSELV TASVNLTTKF ETRDNVDFWG NHLTQSHPEV LGTTIKSPDK 600
VNCVAKPNAY NSGDMHNYCI NYGNCELPVE SSNQGSLPFH NYSKVNNSNK RRRFSGTAVY 660
ENPQRESSSS KTVVQQPISE SFLSLVRQES SKPDSLLASI SLLNDKDGTL KAKSEIEEQY 720
VLEKGQNIDG QNLYSNENQN LECATEKSKW EDFSNVDSPM MPRITSVFSL QSQQASEFLP 780
PEVNQLLQDV LKIKPDVKQD SSNTPNKGLP LHCDQSFQKH EREGKIVESS KDFKVQGIFP 840
VPPGSVGINV PTNDLNLKFG KEKQVSSIPQ DVRDSEKMPR ISGFGTLLKT QSDAIITQQL 900
VKDKLRATTQ NLGSFYMQSP LLNSEQKKTI IVQTSKGFLI PLNITNKPGL PVIPGNALPL 960
VNSQGIPASL FVNKKPGMVL TLNNGKLEGV SAVKTEGAPA RGTVTKEPCK TPILKVEPNN 1020
NCLTPGLCSS IGSCLSMKSS SENTLPLKGP YILKPTSSVK AVLIPNMLSE QQSTKLNISD 1080
SVKQQNEIFP KPPLYTFLPD GKQAVFLKCV MPNKTELLKP KLVQNSTYQN IQPKKPEGTP 1140
QRILLKIFNP VLNVTAANNL SVSNSASSLQ KDNVPSNQII GGEQKEPESR DALPFLLDDL 1200
MPANEIVITS TATCPESSEE PICVSDCSES RVLRCKTNCR IERNFNRKKT SKKIFSKTKT 1260
HGSKDSETAF VSRNRNCKRK CRDSYQEPPR RKATLHRKCK EKAKPEDVRE TFGFSRPRLS 1320
KDSIRTLRLF PFSSKQLVKC PRRNQPVVVL NHPDADAPEV VSVMKTIAKF NGHVLKVSLS 1380
KRTINALLKP VCYNPPKTTY DDFSKRHKTF KPVSSVKERF VLKLTLKKTS KNNYQIVKTT 1440
SENILKAKFN CWFCGRVFDN QDTWAGHGQR HLMEATRDWN MLE 1483 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS