CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005667
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sperm-specific antigen 2 
Protein Synonyms/Alias
 Cleavage signal-1 protein; CS-1; Ki-ras-induced actin-interacting protein 
Gene Name
 SSFA2 
Gene Synonyms/Alias
 CS1; KIAA1927; KRAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
77RGNVPNEKIAIWLKDubiquitination[1, 2]
102EQSSSTLKGVLVRNGubiquitination[1, 2]
199DEPDIASKIPSRFFNubiquitination[1, 2]
212FNSSSFAKGIDIKVFubiquitination[1, 2, 3, 4, 5]
278MSSVTSNKETDPPPPubiquitination[1, 2]
297NTANRLMKTLSKLNLubiquitination[2]
301RLMKTLSKLNLCVDKubiquitination[1]
311LCVDKTEKGESSSPSubiquitination[1]
325SPSAEKGKILNVSVIubiquitination[1, 2]
397GTENEQSKETQSHESubiquitination[1]
405ETQSHESKLGEESGIubiquitination[1]
416ESGIVESKLDSDFNIubiquitination[1]
664YTTHHILKSLASIEAubiquitination[1, 2]
785EEEQELEKRVMEHDGubiquitination[1, 2]
797HDGQSLVKSTIFISPubiquitination[1, 2]
808FISPSSVKKEEAPQSubiquitination[1, 2]
809ISPSSVKKEEAPQSEubiquitination[1]
947AAPYSTQKSSVLPLYubiquitination[1, 2]
1137PSTASVGKSKTPLVAubiquitination[2]
1200EVEEGHGKLPSMPAAubiquitination[1]
1212PAAEEMHKNVEQDELubiquitination[1]
1250LAAVSSSKASNSKQDubiquitination[1, 2]
1255SSKASNSKQDYH***ubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 MOD_RES 87 87 Phosphothreonine.
 MOD_RES 92 92 Phosphoserine.
 MOD_RES 270 270 Phosphoserine.
 MOD_RES 330 330 Phosphoserine.
 MOD_RES 367 367 Phosphoserine (By similarity).
 MOD_RES 650 650 Phosphoserine (By similarity).
 MOD_RES 668 668 Phosphoserine.
 MOD_RES 737 737 Phosphoserine.
 MOD_RES 739 739 Phosphoserine.
 MOD_RES 746 746 Phosphoserine.
 MOD_RES 759 759 Phosphoserine.
 MOD_RES 1134 1134 Phosphoserine.
 MOD_RES 1161 1161 Phosphoserine (By similarity).  
Keyword
 Actin-binding; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1259 AA 
Protein Sequence
MDRPLSSSAE AEEELEWQVA SRRRKAWAKC RSSWQASETE DLSTEATTQD EEEDEEEDLP 60
GAQLPAAGGR GNVPNEKIAI WLKDCRTPLG ASLDEQSSST LKGVLVRNGG SFEDDLSLGA 120
EANHLHESDA QIENCNNILA KERRLQFHQK GRSMNSTGSG KSSGTVSSVS ELLELYEEDP 180
EEILYNLGFG RDEPDIASKI PSRFFNSSSF AKGIDIKVFL SAQMQRMEVE NPNYALTSRF 240
RQIEVLTTVA NAFSSLYSQV SGTPLQRIGS MSSVTSNKET DPPPPLTRSN TANRLMKTLS 300
KLNLCVDKTE KGESSSPSPS AEKGKILNVS VIEESGNKND QKSQKIMKKK ESSSMLATVK 360
EEVSGSSAAV TENADSDRIS DEANSNFNQG TENEQSKETQ SHESKLGEES GIVESKLDSD 420
FNISSHSELE NSSELKSVHI STPEKEPCAP LTIPSIRNIM TQQKDSFEME EVQSTEGEAP 480
HVPATYQLGL TKSKRDHLLR TASQHSDSSG FAEDSTDCLS LNHLQVQESL QAMGSSADSC 540
DSETTVTSLG EDLATPTAQD QPYFNESEEE SLVPLQKGLE KAAAVADKRK SGSQDFPQCN 600
TIENTGTKQS TCSPGDHIIE ITEVEEDLFP AETVELLREA SAESDVGKSS ESEFTQYTTH 660
HILKSLASIE AKCSDMSSEN TTGPPSSMDR VNTALQRAQM KVCSLSNQRM GRSLLKSKDL 720
LKQRYLFAKA GYPLRRSQSL PTTLLSPVRV VSSVNVRLSP GKETRCSPPS FTYKYTPEEE 780
QELEKRVMEH DGQSLVKSTI FISPSSVKKE EAPQSEAPRV EECHHGRTPT CSRLAPPPMS 840
QSTCSLHSIH SEWQERPLCE HTRTLSTHSV PNISGATCSA FASPFGCPYS HRHATYPYRV 900
CSVNPPSAIE MQLRRVLHDI RNSLQNLSQY PMMRGPDPAA APYSTQKSSV LPLYENTFQE 960
LQVMRRSLNL FRTQMMDLEL AMLRQQTMVY HHMTEEERFE VDQLQGLRNS VRMELQDLEL 1020
QLEERLLGLE EQLRAVRMPS PFRSSALMGM CGSRSADNLS CPSPLNVMEP VTELMQEQSY 1080
LKSELGLGLG EMGFEIPPGE SSESVFSQAT SESSSVCSGP SHANRRTGVP STASVGKSKT 1140
PLVARKKVFR ASVALTPTAP SRTGSVQTPP DLESSEEVDA AEGAPEVVGP KSEVEEGHGK 1200
LPSMPAAEEM HKNVEQDELQ QVIREIKESI VGEIRREIVS GLLAAVSSSK ASNSKQDYH 1259 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; NAS:ProtInc. 
Interpro
 IPR026648; SSFA2. 
Pfam
  
SMART
  
PROSITE
  
PRINTS