UniProt Accession

 DCTN3_HUMAN; O75935; A6NII7; B2RBM5; Q5T1I5; Q5T1I7; Q5T8G3; Q9BPU8 

Genbank Protein ID

 AF082513; CR541889; AK314730; AL160270; AL450283; AL160270; AL450283; AL160270; AL450283; AL450283; AL450283; AL160270; AL450283; AL160270; AL450283; AL160270; CH471071; CH471071; BC000319; BC003004; BC107697 

Genbank Nucleotide ID

 AAC61280.1; CAG46687.1; BAG37272.1; CAI13143.1; CAI13143.1; CAI13144.1; CAI13144.1; CAI13145.1; CAI13145.1; CAI14178.1; CAI14180.1; CAI14180.1; CAI14181.1; CAI14181.1; CAI14182.1; CAI14182.1; EAW58440.1; EAW58441.1; AAH00319.1; AAH03004.1; AAI07698.1 

Protein Name

 Dynactin subunit 3 

Protein Synonyms/Alias

 Dynactin complex subunit 22 kDa subunit; p22 

Gene Name

 DCTN3 

Gene Synonyms/Alias

 DCTN22 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	GGARGSRKVADGLVK	ubiquitination	[1]
50	ALGNISSKRERVKIL	ubiquitination	[1, 2, 3, 4]
60	RVKILYKKIEDLIKY	ubiquitination	[1]
66	KKIEDLIKYLDPEYI	ubiquitination	[1]
148	VEITEESKALLEEYN	ubiquitination	[5]
156	ALLEEYNKTTMLLSK	ubiquitination	[5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Together with dynein may be involved in spindle assembly and cytokinesis. 

Sequence Annotation

  

Keyword

 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinetochore; Mitosis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 186 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005869; C:dynactin complex; IPI:UniProtKB.
 GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005198; F:structural molecule activity; TAS:ProtInc.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0000910; P:cytokinesis; IDA:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007017; P:microtubule-based process; IEA:Compara.
 GO:0007067; P:mitosis; TAS:ProtInc. 

Interpro

 IPR009991; Dynactin_p22. 

Pfam

 PF07426; Dynactin_p22 

SMART

  

PROSITE

  

PRINTS