UniProt Accession

 TPM4_MOUSE; Q6IRU2 

Genbank Protein ID

 BC023701; BC023827; BC070421 

Genbank Nucleotide ID

 AAH23701.1; AAH23827.1; AAH70421.1 

Protein Name

 Tropomyosin alpha-4 chain 

Protein Synonyms/Alias

 Tropomyosin-4 

Gene Name

 Tpm4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
11	LNSLEAVKRKIQALQ	acetylation	[1, 2]
11	LNSLEAVKRKIQALQ	ubiquitination	[3]
13	SLEAVKRKIQALQQQ	acetylation	[1]
113	EILEMQLKEAKHITD	acetylation	[2]
113	EILEMQLKEAKHITD	ubiquitination	[3]
125	ITDEADRKYEEVARK	acetylation	[2]
153	RAEVSELKCGDLEEE	acetylation	[4]
153	RAEVSELKCGDLEEE	succinylation	[4]
169	KNVTNNLKSLEAASE	acetylation	[4]
169	KNVTNNLKSLEAASE	succinylation	[4]
169	KNVTNNLKSLEAASE	ubiquitination	[3]
177	SLEAASEKYSEKEDK	acetylation	[1]
177	SLEAASEKYSEKEDK	ubiquitination	[3]
181	ASEKYSEKEDKYEEE	acetylation	[1]
184	KYSEKEDKYEEEIKL	acetylation	[1, 5]
197	KLLSDKLKEAETRAE	ubiquitination	[3]
215	RTVSKLEKTIDDLEE	acetylation	[1, 2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium (By similarity). 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 177 177 N6-acetyllysine (By similarity).
 MOD_RES 215 215 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Actin-binding; Calcium; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Muscle protein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 248 AA 

Protein Sequence

Gene Ontology

 GO:0030863; C:cortical cytoskeleton; IDA:MGI.
 GO:0002102; C:podosome; IDA:MGI.
 GO:0001725; C:stress fiber; ISO:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006979; P:response to oxidative stress; IEA:Compara. 

Interpro

 IPR000533; Tropomyosin. 

Pfam

 PF00261; Tropomyosin 

SMART

  

PROSITE

 PS00326; TROPOMYOSIN 

PRINTS

 PR00194; TROPOMYOSIN.