CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010100
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex 
Protein Synonyms/Alias
 Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 
Gene Name
 pdhC 
Gene Synonyms/Alias
 MPN_391; MP447 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
6**MANEFKFTDVGEGacetylation[1]
18GEGLHEGKVTEILKKacetylation[1]
254VFNGRYDKERNLIVLacetylation[1]
295KSVVDIAKDIVDLANacetylation[1]
310RARSKQIKLPDLSKGacetylation[1]
387ADVGRFGKEIAKQIEacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). 
Sequence Annotation
 DOMAIN 1 76 Lipoyl-binding.
 ACT_SITE 374 374 Potential.
 MOD_RES 43 43 N6-lipoyllysine (By similarity).  
Keyword
 Acyltransferase; Complete proteome; Glycolysis; Lipoyl; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 402 AA 
Protein Sequence
MANEFKFTDV GEGLHEGKVT EILKKVGDTI KVDEALFVVE TDKVTTELPS PYAGVITAIT 60
TNVGDVVHIG QVMAVIDDGA GAAAPAAPQP VSAPAPAPTP TFTPTPAPVT TEPVVEEAGA 120
SVVGEIKVSN SVFPIFGVQP SAPQPTPAPV VQPTSAPTPT PAPASAAAPS GEETIAITTM 180
RKAIAEAMVK SHENIPATIL TFYVNATKLK QYRESVNGLA LSKYNMKISF FAFFVKAIVN 240
ALKKFPVFNG RYDKERNLIV LNKDVNVGIA VDTPDGLIVP NIKQAQTKSV VDIAKDIVDL 300
ANRARSKQIK LPDLSKGTIS VTNFGSLGAA FGTPIIKHPE MCIVATGNME ERVVRAEGGV 360
AVHTILPLTI AADHRWVDGA DVGRFGKEIA KQIEELIDLE VA 402 
Gene Ontology
 GO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS