UniProt Accession

 PHP14_HUMAN; Q9NRX4; B1AMX0; B1AMX1; Q9H0Y3 

Genbank Protein ID

 AF393504; AF164795; AF285119; AL136644; AL355987; AL355987; CH471090; BC024648; BQ922335 

Genbank Nucleotide ID

 AAN52504.1; AAF80759.1; AAG01156.1; CAB66579.1; CAI12692.1; CAI12693.1; EAW88289.1; AAH24648.1 

Protein Name

 14 kDa phosphohistidine phosphatase 

Protein Synonyms/Alias

 Phosphohistidine phosphatase 1; Protein janus-A homolog 

Gene Name

 PHPT1 

Gene Synonyms/Alias

 PHP14; CGI-202; HSPC141 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
59	YHADIYDKVSGDMQK	ubiquitination	[1, 2, 3, 4]
66	KVSGDMQKQGCDCEC	ubiquitination	[5, 6]
112	STEKIKAKYPDYEVT	ubiquitination	[7]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Exhibits phosphohistidine phosphatase activity. 

Sequence Annotation

 REGION 94 96 Substrate binding.
 ACT_SITE 53 53 Proton acceptor.
 BINDING 21 21 Substrate.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 93 93 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Phosphoprotein; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 125 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:BHF-UCL.
 GO:0019855; F:calcium channel inhibitor activity; IDA:BHF-UCL.
 GO:0008969; F:phosphohistidine phosphatase activity; IDA:BHF-UCL.
 GO:0004721; F:phosphoprotein phosphatase activity; IEA:UniProtKB-KW.
 GO:2000984; P:negative regulation of ATP citrate synthase activity; IDA:BHF-UCL.
 GO:0051350; P:negative regulation of lyase activity; IDA:BHF-UCL.
 GO:0050860; P:negative regulation of T cell receptor signaling pathway; IMP:BHF-UCL.
 GO:0035971; P:peptidyl-histidine dephosphorylation; IDA:BHF-UCL.
 GO:2000147; P:positive regulation of cell motility; IMP:BHF-UCL.
 GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Compara.
 GO:2000249; P:regulation of actin cytoskeleton reorganization; IMP:BHF-UCL. 

Interpro

 IPR007702; Ocnus. 

Pfam

 PF05005; Ocnus 

SMART

  

PROSITE

  

PRINTS