UniProt Accession

 PARN_MOUSE; Q8VDG3; Q8C7N6; Q9DC46 

Genbank Protein ID

 AK004572; AK045181; BC021899 

Genbank Nucleotide ID

 BAB23382.1; BAC32249.1; AAH21899.1 

Protein Name

 Poly(A)-specific ribonuclease PARN 

Protein Synonyms/Alias

 Polyadenylate-specific ribonuclease 

Gene Name

 Parn 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
508	TYAEYVGKKQEGKQV	acetylation	[1]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (By similarity). 

Sequence Annotation

 DOMAIN 171 238 R3H.
 METAL 28 28 Divalent metal cation; catalytic (By
 METAL 30 30 Divalent metal cation; catalytic (By
 METAL 285 285 Divalent metal cation; catalytic (By
 METAL 375 375 Divalent metal cation; catalytic (By
 MOD_RES 213 213 N6-acetyllysine (By similarity).
 MOD_RES 492 492 N6-acetyllysine (By similarity).
 MOD_RES 543 543 Phosphoserine; by MAPKAPK2 (By
 MOD_RES 573 573 Phosphoserine.
 MOD_RES 575 575 Phosphoserine.
 MOD_RES 605 605 Phosphoserine (By similarity).
 MOD_RES 609 609 Phosphoserine (By similarity).
 MOD_RES 613 613 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nonsense-mediated mRNA decay; Nuclease; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 624 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IEA:EC.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:MGI.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR014789; PolyA-riboNase_RNA_binding.
 IPR001374; R3H_ss-bd.
 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 

Pfam

 PF04857; CAF1
 PF01424; R3H
 PF08675; RNA_bind 

SMART

  

PROSITE

 PS51061; R3H 

PRINTS