CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011088
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Antigen peptide transporter 1 
Protein Synonyms/Alias
 APT1; ATP-binding cassette sub-family B member 2; Peptide supply factor 1; Peptide transporter PSF1; PSF-1; Peptide transporter TAP1; Peptide transporter involved in antigen processing 1; Really interesting new gene 4 protein 
Gene Name
 TAP1 
Gene Synonyms/Alias
 ABCB2; PSF1; RING4; Y3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
449NEEGEAQKFREKLQEubiquitination[1, 2]
458REKLQEIKTLNQKEAubiquitination[2]
537KAVGSSEKIFEYLDRubiquitination[2]
666IAYGLTQKPTMEEITubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. 
Sequence Annotation
 DOMAIN 247 530 ABC transmembrane type-1.
 DOMAIN 563 802 ABC transporter.
 NP_BIND 598 605 ATP (Potential).
 REGION 435 480 Involved in peptide-binding site.
 REGION 513 547 Involved in peptide-binding site.  
Keyword
 3D-structure; Adaptive immunity; ATP-binding; Complete proteome; Endoplasmic reticulum; Host-virus interaction; Immunity; Membrane; Nucleotide-binding; Peptide transport; Polymorphism; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 808 AA 
Protein Sequence
MAELLASAGS ACSWDFPRAP PSFPPPAASR GGLGGTRSFR PHRGAESPRP GRDRDGVRVP 60
MASSRCPAPR GCRCLPGASL AWLGTVLLLL ADWVLLRTAL PRIFSLLVPT ALPLLRVWAV 120
GLSRWAVLWL GACGVLRATV GSKSENAGAQ GWLAALKPLA AALGLALPGL ALFRELISWG 180
APGSADSTRL LHWGSHPTAF VVSYAAALPA AALWHKLGSL WVPGGQGGSG NPVRRLLGCL 240
GSETRRLSLF LVLVVLSSLG EMAIPFFTGR LTDWILQDGS ADTFTRNLTL MSILTIASAV 300
LEFVGDGIYN NTMGHVHSHL QGEVFGAVLR QETEFFQQNQ TGNIMSRVTE DTSTLSDSLS 360
ENLSLFLWYL VRGLCLLGIM LWGSVSLTMV TLITLPLLFL LPKKVGKWYQ LLEVQVRESL 420
AKSSQVAIEA LSAMPTVRSF ANEEGEAQKF REKLQEIKTL NQKEAVAYAV NSWTTSISGM 480
LLKVGILYIG GQLVTSGAVS SGNLVTFVLY QMQFTQAVEV LLSIYPRVQK AVGSSEKIFE 540
YLDRTPRCPP SGLLTPLHLE GLVQFQDVSF AYPNRPDVLV LQGLTFTLRP GEVTALVGPN 600
GSGKSTVAAL LQNLYQPTGG QLLLDGKPLP QYEHRYLHRQ VAAVGQEPQV FGRSLQENIA 660
YGLTQKPTME EITAAAVKSG AHSFISGLPQ GYDTEVDEAG SQLSGGQRQA VALARALIRK 720
PCVLILDDAT SALDANSQLQ VEQLLYESPE RYSRSVLLIT QHLSLVEQAD HILFLEGGAI 780
REGGTHQQLM EKKGCYWAMV QAPADAPE 808 
Gene Ontology
 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IBA:RefGenome.
 GO:0042825; C:TAP complex; IDA:UniProtKB.
 GO:0043531; F:ADP binding; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0042288; F:MHC class I protein binding; IBA:RefGenome.
 GO:0015421; F:oligopeptide-transporting ATPase activity; IBA:RefGenome.
 GO:0042605; F:peptide antigen binding; NAS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0046978; F:TAP1 binding; ISS:UniProtKB.
 GO:0046979; F:TAP2 binding; IBA:RefGenome.
 GO:0046980; F:tapasin binding; IBA:RefGenome.
 GO:0019885; P:antigen processing and presentation of endogenous peptide antigen via MHC class I; IMP:UniProtKB.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0046967; P:cytosol to ER transport; IMP:UniProtKB.
 GO:0006952; P:defense response; IEA:Compara.
 GO:0019060; P:intracellular transport of viral proteins in host cell; IMP:UniProtKB.
 GO:0001916; P:positive regulation of T cell mediated cytotoxicity; IBA:RefGenome. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR013305; ABC_B2.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR005293; Ag_transporter2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS
 PR01896; TAP1PROTEIN.