CPLM-006850

Genbank Nucleotide ID

Transcription initiation factor TFIID subunit 5

Protein Synonyms/Alias

TAFII-90; TBP-associated factor 5; TBP-associated factor 90 kDa

TAF90; YBR198C; YBR1410

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
21	THQPQPVKNQRTNNA	ubiquitination	[1]
93	RTLTPQNKQSPANTK	ubiquitination	[1]
103	PANTKTGKFPEQSSI	acetylation	[2]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.

DOMAIN 56 88 LisH.
REPEAT 464 503 WD 1.
REPEAT 523 562 WD 2.
REPEAT 565 604 WD 3.
REPEAT 607 646 WD 4.
REPEAT 649 688 WD 5.
REPEAT 692 731 WD 6.
MOD_RES 299 299 Phosphoserine.
MOD_RES 411 411 Phosphoserine.
MOD_RES 415 415 Phosphoserine.
MOD_RES 787 787 Phosphoserine.

3D-structure; Coiled coil; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000124; C:SAGA complex; IDA:SGD.
GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD.
GO:0005669; C:transcription factor TFIID complex; IDA:SGD.
GO:0003682; F:chromatin binding; IDA:SGD.
GO:0032947; F:protein complex scaffold; IMP:SGD.
GO:0043130; F:ubiquitin binding; IDA:SGD.
GO:0016573; P:histone acetylation; IDA:SGD.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IC:SGD.

IPR020472; G-protein_beta_WD-40_rep.
IPR006594; LisH_dimerisation.
IPR013720; LisH_dimerisation_subgr.
IPR007582; TFIID-su_WD40-assoc_reg.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PF08513; LisH
PF04494; TFIID_90kDa
PF00400; WD40

SM00667; LisH
SM00320; WD40

PS50896; LISH
PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00320; GPROTEINBRPT.