CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022601
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromatin assembly factor 1 subunit A 
Protein Synonyms/Alias
 CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150 
Gene Name
 Chaf1a 
Gene Synonyms/Alias
 Caip150 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
446TLAGSCGKFAPFEIKacetylation[1, 2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). 
Sequence Annotation
 REGION 1 31 Binds PCNA (By similarity).
 REGION 176 327 Binds CBX1 and CBX3 chromo shadow
 REGION 621 657 Necessary for homodimerization and
 REGION 639 911 Binds to p60 (By similarity).
 MOTIF 217 230 PxVxL motif.
 MOD_RES 190 190 Phosphoserine (By similarity).
 MOD_RES 208 208 Phosphoserine (By similarity).
 MOD_RES 293 293 Phosphoserine (By similarity).
 MOD_RES 838 838 Phosphothreonine (By similarity).  
Keyword
 3D-structure; Cell cycle; Chaperone; Complete proteome; DNA damage; DNA repair; DNA replication; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 911 AA 
Protein Sequence
MLEEPEAATR TAAAVDCKDR PGFPVKRLIQ ARLPFKRLNL VPKEKVEEDT SPKAAVESKV 60
PDLQLSLGTF ESQCHTGSHV GLSTKLVGGQ GPIDSFLRAT IKPVPSVVII DLTENCSDIP 120
DSPEGHSELS PDTAGVVTTV EGAAKQQEHS AAELCLLETP SDITCHMEEE PGSPGDPKRT 180
GDCQAGSLQS CPELTPGSRT CPTKELSSWS KAGDLLFIEK VPVVVLEDIL ATKPSIASLP 240
MMSLDRSVTS ESEILESCPE DDSILSHSST NSSSPTSSPE GPSTPPEHRG GRSSPSTPAC 300
RVAKNFVKGS TEKGRSKLHR DREQQREEKE KLREEIRRAK EEARKKKEEE KELKEKERRE 360
KREKDEKEKA EKQRLKEEKR KERQEALEAK LEEKRKKEEE KRLREEEKRL REEEKRIKAE 420
KAEITRFFQK PKTPQAPKTL AGSCGKFAPF EIKEHMVLAP RCRAALDQDL CDQLDQLLQQ 480
QSVASTFLSD LKSRLPLRSG PTRVCGHDTD IMNRDVVIVE SSKVDGVSER KKFGRMKLLQ 540
FSENHRPAYW GTWNKKTAII RPRNPWAQDK DLLDYEVDSD DEWEEEEPGE SLSHSEGDED 600
DDVGEDEDED DGFFVPHGYL SEDEGVTEEC ADPENHKVHQ KLKAKEWDEL LAKGKRFRVL 660
QPVHVGCVWA SEAANCTSSD LKLLQQFTAC LLDVASPDEP EPGASRREKR DQHILAQLLP 720
LLHGNVNGSK VIIHEFQEQC RRGLLTLPSP TPHLQMPNLE DAVAVPSKAR LKRLISENSA 780
YEKRPNFRMC WYVHPEVLKS FGQECLPVPC QWTYITTMPS APREDSGSAS TEGPGQSTPM 840
LLKRKPAATM CITQFMKKRR YDGQVGSGDM DGFQADTEED EEDDTDCMII DVPDVGSDVS 900
EAPIPAPTLC K 911 
Gene Ontology
 GO:0033186; C:CAF-1 complex; ISS:UniProtKB.
 GO:0071778; C:WINAC complex; IEA:Compara.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0031497; P:chromatin assembly; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR021644; CAF-1_p150.
 IPR022043; CAF1A. 
Pfam
 PF11600; CAF-1_p150
 PF12253; CAF1A 
SMART
  
PROSITE
  
PRINTS