CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008914
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E4 ubiquitin-protein ligase UFD2 
Protein Synonyms/Alias
 Ubiquitin conjugation factor E4; Ubiquitin fusion degradation protein 2; UB fusion protein 2 
Gene Name
 UFD2 
Gene Synonyms/Alias
 YDL190C; D1255 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
214KPQDFERKTILGPILubiquitination[1]
362TRLNSDFKEADAFYDubiquitination[1]
447SKMEKALKTTESLRFubiquitination[1]
637SSSQFYDKFNSRYSIubiquitination[1]
721PTREEEDKELQTRLAubiquitination[1]
735ASASRQAKSSCGLADubiquitination[1]
743SSCGLADKSMKLFEIubiquitination[1]
790KCGELKVKDPQSYSFubiquitination[1]
845AVDILGRKTGLASPEubiquitination[1]
913NIDRSTIKAHLLSDSubiquitination[1]
930PFNRMPLKLEDVTPNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 E4 ubiquitin chain-elongation enzyme specifically involved in polyubiquitin chain assembly. Binds to CDC48 and elongates mono- and diubiquitinated ERAD substrates presented by the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex to a chain length of 4 to 6 ubiquitin moieties. Delivers these polyubiquitinated substrates to RAD23 and DSK2, which target them to the proteasome. Has E3 ubiquitin-protein ligase activity, accepting ubiquitin from its cognate E2 ubiquitin-conjugating enzyme UBC4. Enhances ubiquitination at 'Lys-48', but not at 'Lys-29' of the Ub moiety. Promotes ubiquitin chain elongation at 'Lys-48' on the DOA10 substrate PEX29. Also involved in the proteolytic processing of the ER-bound transcription factor SPT23. 
Sequence Annotation
 DOMAIN 884 947 U-box.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Ligase; Nucleus; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 961 AA 
Protein Sequence
MTAIEDILQI TTDPSDTRGY SLLKSEEVPQ GSTLGVDFID TLLLYQLTEN EKLDKPFEYL 60
NDCFRRNQQQ KRITKNKPNA ESLHSTFQEI DRLVIGYGVV ALQIENFCMN GAFINYITGI 120
VSNVNSYTDF LSQIIQRAIL EGTALDLLNA VFPTLLEYCN KHVSHFDLNE SVIYNNVLTI 180
FELFVTFKPI AEIFTKIDGF FADYSCKPQD FERKTILGPI LSLSPIEAAV AIRNYGDNLL 240
RSKQQTAMIH ESLQAEHKVV IDRLFFIVDK LVRGSLNSRT DMISYFAHIA NKNHLRRADH 300
PPFKELSSNG FMSNITLLLV RFSQPFLDIS YKKIDKIDAN YFNNPSLFID LSGETRLNSD 360
FKEADAFYDK NRKTADSKPN FISDCFFLTL TYLHYGLGGT LSFEEKMGSE IKALKEEIEK 420
VKKIAANHDV FARFITAQLS KMEKALKTTE SLRFALQGFF AHRSLQLEVF DFICGASTFL 480
IRVVDPEHEF PFKQIKLPLI PDQIGVENVD NADFLRAHAP VPFKYYPEFV VEGPVNYSLY 540
ISKYQTSPIF RNPRLGSFVE FTTMVLRCPE LVSNPHLKGK LVQLLSVGAM PLTDNSPGFM 600
MDIFEHDELV NKNLLYALLD FYVIVEKTGS SSQFYDKFNS RYSISIILEE LYYKIPSYKN 660
QLIWQSQNNA DFFVRFVARM LNDLTFLLDE GLSNLAEVHN IQNELDNRAR GAPPTREEED 720
KELQTRLASA SRQAKSSCGL ADKSMKLFEI YSKDIPAAFV TPEIVYRLAS MLNYNLESLV 780
GPKCGELKVK DPQSYSFNPK DLLKALTTVY INLSEQSEFI SAVAKDERSF NRNLFVRAVD 840
ILGRKTGLAS PEFIEKLLNF ANKAEEQRKA DEEEDLEYGD VPDEFLDPLM YTIMKDPVIL 900
PASKMNIDRS TIKAHLLSDS TDPFNRMPLK LEDVTPNEEL RQKILCFKKQ KKEEAKHKAS 960
E 961 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0000151; C:ubiquitin ligase complex; IEA:InterPro.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IDA:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD. 
Interpro
 IPR019474; Ub_conjug_fac_E4_core.
 IPR003613; Ubox_domain.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF04564; U-box
 PF10408; Ufd2P_core 
SMART
 SM00504; Ubox 
PROSITE
  
PRINTS