CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017716
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box only protein 18 
Protein Synonyms/Alias
 F-box DNA helicase 1 
Gene Name
 FBXO18 
Gene Synonyms/Alias
 FBH1; FBX18 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
308PLFIPWKKLYHRYLMubiquitination[1]
352IRYTATTKCSPSVDPubiquitination[1, 2]
598NSVLAEGKGGFIRAKubiquitination[1]
662RLWDNMRKLGECTEEubiquitination[1]
828IHLIGGIKSFGLDRIubiquitination[1]
893AKIAVVEKYNIRIPEubiquitination[1]
927LGTVHKAKGLEFDTVubiquitination[1]
942HVLDDFVKVPCARHNubiquitination[1]
1042PITYSNRKENKGGYLubiquitination[1]
1045YSNRKENKGGYLCHSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 DNA-dependent ATPase. Unwinds double-stranded DNA in a 3' to 5' direction. 
Sequence Annotation
 DOMAIN 138 184 F-box.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1043 AA 
Protein Sequence
MSYEVTSGCH WTCQVPESCD NGLHCAGPLG HLHRRCQRTS AHLLVFTEHA EMRRFKRKHL 60
TAIDCQHLAR SHLAVTQPFG QRWTNRDPNH GLYPKPRTKR GSRGQGSQRC IPEFFLAGKQ 120
PCTNDMAKSN SVGQDSCQDS EGDMIFPAES SCALPQEGSA GPGSPGSAPP SRKRSWSSEE 180
ESNQATGTSR WDGVSKKAPR HHLSVPCTRP REARQEAEDS TSRLSAESGE TDQDAGDVGP 240
DPIPDSYYGL LGTLPCQEAL SHICSLPSEV LRHVFAFLPV EDLYWNLSLV CHLWREIISD 300
PLFIPWKKLY HRYLMNEEQA VSKVDGILSN CGIEKESDLC VLNLIRYTAT TKCSPSVDPE 360
RVLWSLRDHP LLPEAEACVR QHLPDLYAAA GGVNIWALVA AVVLLSSSVN DIQRLLFCLR 420
RPSSTVTMPD VTETLYCIAV LLYAMREKGI NISNRIHYNI FYCLYLQENS CTQATKVKEE 480
PSVWPGKKTI QLTHEQQLIL NHKMEPLQVV KIMAFAGTGK TSTLVKYAEK WSQSRFLYVT 540
FNKSIAKQAE RVFPSNVICK TFHSMAYGHI GRKYQSKKKL NLFKLTPFMV NSVLAEGKGG 600
FIRAKLVCKT LENFFASADE ELTIDHVPIW CKNSQGQRVM VEQSEKLNGV LEASRLWDNM 660
RKLGECTEEA HQMTHDGYLK LWQLSKPSLA SFDAIFVDEA QDCTPAIMNI VLSQPCGKIF 720
VGDPHQQIYT FRGAVNALFT VPHTHVFYLT QSFRFGVEIA YVGATILDVC KRVRKKTLVG 780
GNHQSGIRGD AKGQVALLSR TNANVFDEAV RVTEGEFPSR IHLIGGIKSF GLDRIIDIWI 840
LLQPEEERRK QNLVIKDKFI RRWVHKEGFS GFKRYVTAAE DKELEAKIAV VEKYNIRIPE 900
LVQRIEKCHI EDLDFAEYIL GTVHKAKGLE FDTVHVLDDF VKVPCARHNL PQLPHFRVES 960
FSEDEWNLLY VAVTRAKKRL IMTKSLENIL TLAGEYFLQA ELTSNVLKTG VVRCCVGQCN 1020
NAIPVDTVLT MKKLPITYSN RKENKGGYLC HSCAEQRIGP LAFLTASPEQ VRAMERTVEN 1080
IVLPRHEALL FLVF 1094 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR014017; DNA_helicase_UvrD-like_C.
 IPR000212; DNA_helicase_UvrD/REP.
 IPR001810; F-box_dom_cyclin-like.
 IPR027417; P-loop_NTPase.
 IPR014016; UvrD-like_ATP-bd. 
Pfam
 PF00580; UvrD-helicase
 PF13361; UvrD_C 
SMART
 SM00256; FBOX 
PROSITE
 PS50181; FBOX 
PRINTS