UniProt Accession

 NPL4_YEAST; P33755; D6VQG6 

Genbank Protein ID

 X72224; X74437; Z36039; BK006936 

Genbank Nucleotide ID

 CAA51026.1; CAA52450.1; CAA85131.1; DAA07286.1 

Protein Name

 Nuclear protein localization protein 4 

Protein Synonyms/Alias

 HMG-CoA reductase degradation protein 4 

Gene Name

 NPL4 

Gene Synonyms/Alias

 HRD4; YBR170C; YBR1231 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
218	WPRGICSKCQPSAIT	ubiquitination	[1]
269	YMYGSYSKYDNTPLG	ubiquitination	[1]
342	GDGSVFCKRHKDSFF	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD. 

Sequence Annotation

  

Keyword

 Complete proteome; Cytoplasm; Endoplasmic reticulum; Membrane; mRNA transport; Nucleus; Protein transport; Reference proteome; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 580 AA 

Protein Sequence

Gene Ontology

 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:SGD.
 GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IDA:SGD.
 GO:0000837; C:Doa10p ubiquitin ligase complex; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0071629; P:cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0071712; P:ER-associated misfolded protein catabolic process; IMP:SGD.
 GO:0072671; P:mitochondria-associated protein catabolic process; IMP:SGD.
 GO:0051228; P:mitotic spindle disassembly; IMP:SGD.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:1900182; P:positive regulation of protein localization to nucleus; IMP:SGD.
 GO:0030970; P:retrograde protein transport, ER to cytosol; IMP:SGD. 

Interpro

 IPR007717; NPL4.
 IPR007716; NPL4_Zn-bd_put.
 IPR016563; PolyUb_recognition_cplx_Npl4. 

Pfam

 PF05021; NPL4
 PF05020; zf-NPL4 

SMART

  

PROSITE

  

PRINTS