UniProt Accession

 H2B1A_HUMAN; Q96A08; B2R544; Q6NZ98; Q6NZA0; Q6NZA1 

Genbank Protein ID

 AF397301; AF531284; AK312055; AL512384; CH471087; BC066238; BC066239; BC066240; BC066241; BC066242; BC066243 

Genbank Nucleotide ID

 AAK84040.1; AAN06684.1; BAG34991.1; CAC44615.1; EAW55490.1; AAH66238.1; AAH66239.1; AAH66240.1; AAH66241.1; AAH66242.1; AAH66243.1 

Protein Name

 Histone H2B type 1-A 

Protein Synonyms/Alias

 Histone H2B, testis; Testis-specific histone H2B 

Gene Name

 HIST1H2BA 

Gene Synonyms/Alias

 TSH2B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
18	ISKKGFKKAVVKTQK	acetylation	[1]
22	GFKKAVVKTQKKEGK	acetylation	[1]
48	IYIYKVLKQVHPDTG	ubiquitination	[2]
110	LLPGELAKHAVSEGT	ubiquitination	[2, 3]
118	HAVSEGTKAVTKYTS	ubiquitination	[2]
122	EGTKAVTKYTSSK**	ubiquitination	[2, 3, 4]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. 

Sequence Annotation

 MOD_RES 2 2 N-acetylproline (By similarity).
 MOD_RES 7 7 N6-acetyllysine; alternate.
 MOD_RES 7 7 N6-crotonyl-L-lysine; alternate.
 MOD_RES 13 13 N6-acetyllysine; alternate (By
 MOD_RES 13 13 N6-crotonyl-L-lysine; alternate.
 MOD_RES 14 14 N6-acetyllysine; alternate.
 MOD_RES 14 14 N6-crotonyl-L-lysine; alternate.
 MOD_RES 17 17 N6-acetyllysine; alternate.
 MOD_RES 17 17 N6-crotonyl-L-lysine; alternate.
 MOD_RES 18 18 N6-acetyllysine; alternate (By
 MOD_RES 18 18 N6-crotonyl-L-lysine; alternate.
 MOD_RES 22 22 N6-acetyllysine; alternate.
 MOD_RES 22 22 N6-crotonyl-L-lysine; alternate.
 MOD_RES 25 25 N6-acetyllysine; alternate (By
 MOD_RES 25 25 N6-crotonyl-L-lysine; alternate.
 MOD_RES 36 36 N6-crotonyl-L-lysine; alternate.
 MOD_RES 38 38 Phosphoserine (By similarity).
 MOD_RES 44 44 Phosphotyrosine (By similarity).
 MOD_RES 48 48 N6-methyllysine (By similarity).
 MOD_RES 59 59 N6,N6-dimethyllysine (By similarity).
 MOD_RES 110 110 N6-methyllysine (By similarity).
 MOD_RES 114 114 Phosphoserine (By similarity).
 CROSSLNK 36 36 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 122 122 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 127 AA 

Protein Sequence

Gene Ontology

 GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
 GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0071229; P:cellular response to acid; IEA:Compara.
 GO:0006954; P:inflammatory response; IEA:Compara.
 GO:0071674; P:mononuclear cell migration; IEA:Compara.
 GO:0006334; P:nucleosome assembly; IEA:InterPro.
 GO:0031639; P:plasminogen activation; IEA:Compara.
 GO:0051099; P:positive regulation of binding; IEA:Compara. 

Interpro

 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR000558; Histone_H2B. 

Pfam

 PF00125; Histone 

SMART

 SM00427; H2B 

PROSITE

 PS00357; HISTONE_H2B 

PRINTS

 PR00621; HISTONEH2B.