UniProt Accession

 AP2B1_HUMAN; P63010; A6NJP3; P21851; Q96J19 

Genbank Protein ID

 M34175; AC006237; CH471147; BC006201 

Genbank Nucleotide ID

 AAA35583.1; EAW80133.1; AAH06201.1 

Protein Name

 AP-2 complex subunit beta 

Protein Synonyms/Alias

 AP105B; Adapter-related protein complex 2 beta subunit; Adaptor protein complex AP-2 subunit beta; Beta-2-adaptin; Beta-adaptin; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor HA2/AP2 adaptin beta subunit 

Gene Name

 AP2B1 

Gene Synonyms/Alias

 ADTB2; CLAPB1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MTDSKYFTTNKK	ubiquitination	[1]
117	MGCIRVDKITEYLCE	ubiquitination	[2]
131	EPLRKCLKDEDPYVR	ubiquitination	[3]
538	STDPVTAKEVVLSEK	ubiquitination	[2]
733	AVWLPAVKAKGLEIS	ubiquitination	[1, 2]
735	WLPAVKAKGLEISGT	ubiquitination	[1]
869	NELQFQIKECHLNAD	ubiquitination	[1]
881	NADTVSSKLQNNNVY	ubiquitination	[1]
892	NNVYTIAKRNVEGQD	ubiquitination	[1, 3]
931	PNYTLSLKCRAPEVS	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin- associated sorting proteins (CLASPs) are recognized by their [DE]- X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. 

Sequence Annotation

 REGION 841 937 Interaction with ARRB1.
 MOD_RES 265 265 N6-acetyllysine.
 MOD_RES 737 737 Phosphotyrosine; by SRC.
 MOD_RES 888 888 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cell membrane; Coated pit; Complete proteome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 937 AA 

Protein Sequence

Gene Ontology

 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0030669; C:clathrin-coated endocytic vesicle membrane; TAS:Reactome.
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0048268; P:clathrin coat assembly; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
 GO:0007268; P:synaptic transmission; TAS:Reactome.
 GO:0016032; P:viral reproduction; TAS:Reactome. 

Interpro

 IPR026739; AP_beta.
 IPR016342; AP_complex_bsu_1_2_4.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR015151; B-adaptin_app_sub_C.
 IPR012295; Beta2_adaptin/TBP_C_dom.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR013037; Clathrin_b-adaptin_app_Ig-like.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like. 

Pfam

 PF01602; Adaptin_N
 PF02883; Alpha_adaptinC2
 PF09066; B2-adapt-app_C 

SMART

 SM00809; Alpha_adaptinC2
 SM01020; B2-adapt-app_C 

PROSITE

  

PRINTS