CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024355
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Targeting protein for Xklp2 
Protein Synonyms/Alias
  
Gene Name
 Tpx2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
75QGFVTPLKAVDNTYHacetylation[1]
128SIRLSAQKDLEQKEKacetylation[1, 2]
307TRGCTIIKPFNLSKGacetylation[1, 2]
375QTPILQTKYRTRAVTacetylation[1]
465HSRPCPTKILEDVVGacetylation[2]
523PHYGVPYKPHIAEARacetylation[1]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation (By similarity). 
Sequence Annotation
 MOD_RES 72 72 Phosphothreonine (By similarity).
 MOD_RES 294 294 Phosphoserine (By similarity).
 MOD_RES 307 307 N6-acetyllysine (By similarity).
 MOD_RES 312 312 Phosphoserine (By similarity).
 MOD_RES 340 340 Phosphothreonine (By similarity).
 MOD_RES 369 369 Phosphothreonine (By similarity).
 MOD_RES 486 486 Phosphoserine (By similarity).
 MOD_RES 737 737 Phosphoserine.  
Keyword
 Acetylation; Apoptosis; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 745 AA 
Protein Sequence
MSQVPTTYSF DAPTDFINFS SLDAEEDTEN IDSWFDEKAN LENKFLRQRG IGEPFQGKNS 60
LRKAKLQQGF VTPLKAVDNT YHKETEKENL QKQSIPSNDC SSLDAKRAVS GNTPVQPQRR 120
SIRLSAQKDL EQKEKNHVAS VEMKAKRCVA PATDCPPQKR MKVSHKKKLE EEEEGSAPAT 180
SRKNERETLE KAKGKHTVPG VPPAREKVLK STEEQEIEKR LRMQQEVVEL RRKNEEFKKL 240
ALAGPGQPVK KSTSQVTKTV DFHFLTDERI KQHPKNQEEY KEVNFMSELR KHSSTPARGT 300
RGCTIIKPFN LSKGKKRTFD EAASTYVPIA QQVEAFHKRT PNRYHLRNKK DESLLPSKSV 360
NKIARDPQTP ILQTKYRTRA VTCKSTAEQE AEELEKLQQY KFKARELDPR IFESGPILPK 420
RAPVKPPTQP VGFDLEIEKR IHERESKKKT EDEQFEFHSR PCPTKILEDV VGVPEKKVIP 480
ATVPKSPVFA LKNRIRVPIK DEEEEKPVVI KAQPVPHYGV PYKPHIAEAR NVEVCPFSFD 540
TRDKERQLQK EKKIKEMQKG EVPKFKALPV PHFDTINLPE KKVKNVTQAE PFSLETDKRG 600
AYKAEMWKHQ LEEEQKQQKD AACFKARPNT VIFQEPFVPK KEKKSLAENP SGSLVQEPFQ 660
LATERRAKER QELEKKMAEV EAWKLQQLEE VRQQEEEQQK EELARLRKEL VHKANPIRKY 720
AAVEVKSSEL PLTVPVSPKF STRFQ 745 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0032147; P:activation of protein kinase activity; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0060236; P:regulation of mitotic spindle organization; IMP:UniProtKB. 
Interpro
 IPR015128; Aurora-A-bd.
 IPR027330; TPX2_central_dom.
 IPR027329; TPX2_dom_C.
 IPR009675; TPX2_fam. 
Pfam
 PF09041; Aurora-A_bind
 PF06886; TPX2
 PF12214; TPX2_importin 
SMART
  
PROSITE
  
PRINTS