UniProt Accession

 E7EUT5_HUMAN; E7EUT5 

Genbank Protein ID

 AC006064 

Genbank Nucleotide ID

  

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase 

Protein Synonyms/Alias

  

Gene Name

 GAPDH 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	EEMRDPSKIKWGDAG	ubiquitination	[1]
11	MRDPSKIKWGDAGAE	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
32	GVFTTMEKAGAHLQG	acetylation	[8, 9]
32	GVFTTMEKAGAHLQG	phosphoglycerylation	[10]
32	GVFTTMEKAGAHLQG	ubiquitination	[1, 3, 4, 6, 7, 8]
42	AHLQGGAKRVIISAP	acetylation	[8]
42	AHLQGGAKRVIISAP	ubiquitination	[1, 3, 4, 5, 7]
64	VMGVNHEKYDNSLKI	acetylation	[8, 9, 11, 12]
64	VMGVNHEKYDNSLKI	phosphoglycerylation	[10]
64	VMGVNHEKYDNSLKI	ubiquitination	[1, 2, 3, 6, 7, 8]
70	EKYDNSLKIISNASC	acetylation	[8]
70	EKYDNSLKIISNASC	phosphoglycerylation	[10]
70	EKYDNSLKIISNASC	ubiquitination	[1, 2, 3, 4, 5, 6, 8]
87	NCLAPLAKVIHDNFG	phosphoglycerylation	[10]
111	HAITATQKTVDGPSG	acetylation	[8, 9, 12]
111	HAITATQKTVDGPSG	ubiquitination	[1, 3, 6, 7, 8, 13]
119	TVDGPSGKLWRDGRG	acetylation	[8, 11, 12]
119	TVDGPSGKLWRDGRG	phosphoglycerylation	[10]
119	TVDGPSGKLWRDGRG	ubiquitination	[1, 2, 3, 4, 6, 7, 8, 13]
140	PASTGAAKAVGKVIP	acetylation	[8, 9]
140	PASTGAAKAVGKVIP	phosphoglycerylation	[10]
140	PASTGAAKAVGKVIP	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 13]
144	GAAKAVGKVIPELNG	acetylation	[8, 12]
144	GAAKAVGKVIPELNG	ubiquitination	[1, 2, 3, 6, 7, 8]
152	VIPELNGKLTGMAFR	acetylation	[8, 12]
152	VIPELNGKLTGMAFR	ubiquitination	[1, 2, 3, 6, 7, 8]
176	DLTCRLEKPAKYDDI	ubiquitination	[1, 5, 6, 8]
179	CRLEKPAKYDDIKKV	acetylation	[8, 9, 11, 12]
179	CRLEKPAKYDDIKKV	phosphoglycerylation	[10]
179	CRLEKPAKYDDIKKV	ubiquitination	[1, 3, 5, 6, 7, 8]
184	PAKYDDIKKVVKQAS	acetylation	[9]
184	PAKYDDIKKVVKQAS	ubiquitination	[1, 3, 5, 7]
185	AKYDDIKKVVKQASE	phosphoglycerylation	[10]
185	AKYDDIKKVVKQASE	ubiquitination	[3, 6]
188	DDIKKVVKQASEGPL	acetylation	[8, 11, 12]
188	DDIKKVVKQASEGPL	phosphoglycerylation	[10]
188	DDIKKVVKQASEGPL	ubiquitination	[1, 2, 3, 4, 6, 7, 8, 13]
196	QASEGPLKGILGYTE	ubiquitination	[1, 3]
259	LMAHMASKE******	acetylation	[8, 9, 12]
259	LMAHMASKE******	ubiquitination	[1, 3, 5, 6, 7, 8]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [11] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [12] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [13] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 260 AA 

Protein Sequence

Gene Ontology

 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.