CPLM-023256

Genbank Nucleotide ID

COP9 signalosome complex subunit 3

Protein Synonyms/Alias

SGN3; Signalosome subunit 3; JAB1-containing signalosome subunit 3

Homo sapiens (Human)

Position	Peptide	Type	References
30	QLCELINKSGELLAK	ubiquitination	[1, 2, 3]
125	LRGIGILKQAIDKMQ	ubiquitination	[1, 3]
243	GKVQQLPKYTSQIVG	ubiquitination	[1, 3, 4, 5, 6, 7]
254	QIVGRFIKPLSNAYH	ubiquitination	[3, 5, 7, 8]
281	ELRNLVNKHSETFTR	ubiquitination	[1, 3, 9, 10]
296	DNNMGLVKQCLSSLY	ubiquitination	[3, 5, 7, 8, 9, 10]
304	QCLSSLYKKNIQRLT	ubiquitination	[3]
305	CLSSLYKKNIQRLTK	ubiquitination	[3]
312	KNIQRLTKTFLTLSL	ubiquitination	[3, 5, 7, 8, 9]
366	SFHDNPEKYNNPAML	ubiquitination	[3]
382	NIDQEMLKCIELDER	ubiquitination	[3]
391	IELDERLKAMDQEIT	ubiquitination	[1, 3, 5, 6, 7, 10, 11]
406	VNPQFVQKSMGSQED	ubiquitination	[1, 3, 5, 7, 11]
418	QEDDSGNKPSSYS**	ubiquitination	[1, 3, 11]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[9] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[11] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

DOMAIN 195 362 PCI.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 410 410 Phosphoserine.
MOD_RES 423 423 Phosphoserine.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Signalosome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:HPA.
GO:0008180; C:signalosome; IDA:UniProtKB.
GO:0010388; P:cullin deneddylation; IDA:UniProtKB.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0009416; P:response to light stimulus; TAS:ProtInc.
GO:0007165; P:signal transduction; TAS:ProtInc.

IPR000717; PCI_dom.
IPR011991; WHTH_DNA-bd_dom.