UniProt Accession

 TLG1_YEAST; Q03322; D6VT92 

Genbank Protein ID

 U33050; AY558178; BK006938 

Genbank Nucleotide ID

 AAB64925.1; AAS56504.1; DAA12302.1 

Protein Name

 T-SNARE affecting a late Golgi compartment protein 1 

Protein Synonyms/Alias

 Syntaxin TLG1 

Gene Name

 TLG1 

Gene Synonyms/Alias

 YDR468C; D8035.11 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
183	GMDGVVNKLARGRRQ	ubiquitination	[1, 2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation. 

Sequence Annotation

 DOMAIN 132 194 t-SNARE coiled-coil homology.
 REGION 1 106 Interaction with VPS51.
 MOD_RES 31 31 Phosphothreonine.
 LIPID 205 205 S-palmitoyl cysteine (Probable).
 LIPID 206 206 S-palmitoyl cysteine (Probable).  

Keyword

 3D-structure; Coiled coil; Complete proteome; Endosome; Golgi apparatus; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 224 AA 

Protein Sequence

Gene Ontology

 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005768; C:endosome; IDA:SGD.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0031201; C:SNARE complex; IPI:SGD.
 GO:0005802; C:trans-Golgi network; IDA:SGD.
 GO:0005484; F:SNAP receptor activity; IDA:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0048193; P:Golgi vesicle transport; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0006906; P:vesicle fusion; IDA:SGD. 

Interpro

 IPR015260; Syntaxin-6_N.
 IPR006012; Syntaxin/epimorphin_CS.
 IPR010989; t-SNARE.
 IPR000727; T_SNARE_dom. 

Pfam

 PF05739; SNARE
 PF09177; Syntaxin-6_N 

SMART

 SM00397; t_SNARE 

PROSITE

 PS00914; SYNTAXIN
 PS50192; T_SNARE 

PRINTS