CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013225
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nitrogen assimilation regulatory protein nac 
Protein Synonyms/Alias
 Nitrogen assimilation control protein 
Gene Name
 nac 
Gene Synonyms/Alias
 b1988; JW1967 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
62VTPTDAGKILYTHARacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression (By similarity). 
Sequence Annotation
 DOMAIN 1 58 HTH lysR-type.
 DNA_BIND 18 37 H-T-H motif (Potential).  
Keyword
 Activator; Complete proteome; DNA-binding; Nitrate assimilation; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 305 AA 
Protein Sequence
MNFRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GELNQQLLIR TKRGVTPTDA 60
GKILYTHARA ILRQCEQAQL AVHNVGQALS GQVSIGFAPG TAASSITMPL LQAVRAEFPE 120
IVIYLHENSG AVLNEKLINH QLDMAVIYEH SPVAGVSSQA LLKEDLFLVG TQDCPGQSVD 180
VNAIAQMNLF LPSDYSAIRL RVDEAFSLRR LTAKVIGEIE SIATLTAAIA SGMGVAVLPE 240
SAARSLCGAV NGWMSRITTP SMSLSLSLNL PARANLSPQA QAVKELLMSV ISSPVMEKRQ 300
WQLVS 305 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:EcoCyc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:EcoCyc.
 GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR005119; LysR_subst-bd.
 IPR000847; Tscrpt_reg_HTH_LysR.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00126; HTH_1
 PF03466; LysR_substrate 
SMART
  
PROSITE
 PS50931; HTH_LYSR 
PRINTS
 PR00039; HTHLYSR.