CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023181
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCR4-NOT transcription complex subunit 6 
Protein Synonyms/Alias
 CCR4 carbon catabolite repression 4-like; Carbon catabolite repressor protein 4 homolog; Cytoplasmic deadenylase 
Gene Name
 CNOT6 
Gene Synonyms/Alias
 CCR4; CCR4a; KIAA1194 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118VLPFELGKLFQLQTLubiquitination[1]
128QLQTLGLKGNPLTQDubiquitination[1]
162DNLSGTAKRITTEQPubiquitination[1, 2, 3]
266YNGFFSPKSRARTMSubiquitination[1]
278TMSEQERKHVDGCAIubiquitination[1]
389EVKNIIDKASRNLKSubiquitination[1]
454TNFSCHGKNGTTNGRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein- coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA. The increase of ligand-dependent ESR1-mediated transcription is much smaller, if any. Mediates cell proliferation and cell survival and prevents cellular senescence. 
Sequence Annotation
 REPEAT 52 73 LRR 1.
 REPEAT 75 96 LRR 2.
 REPEAT 98 120 LRR 3.
 REPEAT 121 143 LRR 4.
 REGION 153 557 Nuclease domain (By similarity).
 ACT_SITE 240 240 By similarity.
 ACT_SITE 491 491 By similarity.
 ACT_SITE 531 531 By similarity.
 METAL 240 240 Magnesium 1 (By similarity).
 METAL 412 412 Magnesium 2 (By similarity).
 METAL 414 414 Magnesium 2 (By similarity).
 METAL 531 531 Magnesium 2 (By similarity).
 MOD_RES 146 146 Phosphothreonine (By similarity).  
Keyword
 Activator; Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Leucine-rich repeat; Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 557 AA 
Protein Sequence
MPKEKYEPPD PRRMYTIMSS EEAANGKKSH WAELEISGKV RSLSASLWSL THLTALHLSD 60
NSLSRIPSDI AKLHNLVYLD LSSNKIRSLP AELGNMVSLR ELHLNNNLLR VLPFELGKLF 120
QLQTLGLKGN PLTQDILNLY QEPDGTRRLL NYLLDNLSGT AKRITTEQPP PRSWIMLQEP 180
DRTRPTALFS VMCYNVLCDK YATRQLYGYC PSWALNWDYR KKAIIQEILS CNADIVSLQE 240
VETEQYYSFF LVELKERGYN GFFSPKSRAR TMSEQERKHV DGCAIFFKTE KFTLVQKHTV 300
EFNQLAMANS EGSEAMLNRV MTKDNIGVAV LLELRKESIE MPSGKPHLGT EKQLILVANA 360
HMHWDPEYSD VKLVQTMMFL SEVKNIIDKA SRNLKSSVLG EFGTIPLVLC ADLNSLPDSG 420
VVEYLSTGGV ETNHKDFKEL RYNESLTNFS CHGKNGTTNG RITHGFKLQS AYESGLMPYT 480
NYTFDFKGII DYIFYSKPQL NTLGILGPLD HHWLVENNIS GCPHPLIPSD HFSLFAQLEL 540
LLPFLPQVNG IHLPGRR 557 
Gene Ontology
 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IDA:UniProtKB.
 GO:0035195; P:gene silencing by miRNA; TAS:UniProtKB.
 GO:0070966; P:nuclear-transcribed mRNA catabolic process, no-go decay; IMP:UniProtKB.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; IMP:UniProtKB.
 GO:2000327; P:positive regulation of ligand-dependent nuclear receptor transcription coactivator activity; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR005135; Endo/exonuclease/phosphatase.
 IPR001611; Leu-rich_rpt.
 IPR003591; Leu-rich_rpt_typical-subtyp. 
Pfam
 PF03372; Exo_endo_phos 
SMART
 SM00369; LRR_TYP 
PROSITE
 PS51450; LRR 
PRINTS