UniProt Accession

 BMI1_HUMAN; P35226; Q16030; Q5T8Z3; Q96F37 

Genbank Protein ID

 L13689; AK313235; AL158211; CH471072; CH471072; CH471072; CH471072; BC011652; AH004292 

Genbank Nucleotide ID

 AAA19873.1; BAG36046.1; CAI15958.1; EAW86148.1; EAW86150.1; EAW86151.1; EAW86154.1; AAH11652.1; AAB27059.1 

Protein Name

 Polycomb complex protein BMI-1 

Protein Synonyms/Alias

 Polycomb group RING finger protein 4; RING finger protein 51 

Gene Name

 BMI1 

Gene Synonyms/Alias

 PCGF4; RNF51 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
224	RNGPLPLKYRVRPTC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. 

Sequence Annotation

 ZN_FING 18 57 RING-type.
 REGION 164 228 Interaction with E4F1.
 MOTIF 81 95 Nuclear localization signal (Potential).
 MOD_RES 251 251 Phosphoserine (By similarity).
 MOD_RES 253 253 Phosphoserine (By similarity).
 MOD_RES 255 255 Phosphoserine (By similarity).  

Keyword

 3D-structure; Chromatin regulator; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 326 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0035102; C:PRC1 complex; IDA:UniProtKB.
 GO:0000151; C:ubiquitin ligase complex; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0030097; P:hemopoiesis; IEP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0048146; P:positive regulation of fibroblast proliferation; IMP:BHF-UCL.
 GO:0051443; P:positive regulation of ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0007379; P:segment specification; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 

Pfam

 PF00097; zf-C3HC4 

SMART

 SM00184; RING 

PROSITE

 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS