CPLM-007542

Genbank Nucleotide ID

Protein Synonyms/Alias

Neural precursor cell expressed developmentally down-regulated protein 5; NEDD-5

Mus musculus (Mouse)

Position	Peptide	Type	References
3	*****MSKQQPTQFI	ubiquitination	[1]
74	IIPGAAEKIERTVQI	ubiquitination	[1]
183	NIVPVIAKADTLTLK	ubiquitination	[1]
225	SDEDEDFKEQTRLLK	ubiquitination	[1]
232	KEQTRLLKASIPFSV	ubiquitination	[1]
249	SNQLIEAKGKKVRGR	ubiquitination	[1]
318	VENEDMNKDQILLEK	ubiquitination	[1]
325	KDQILLEKEAELRRM	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements (By similarity). In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein.

NP_BIND 44 52 GTP.
NP_BIND 183 186 GTP.
REGION 260 270 Important for dimerization (By
BINDING 78 78 GTP.
BINDING 104 104 GTP; via amide nitrogen.
BINDING 241 241 GTP; via amide nitrogen and carbonyl
BINDING 256 256 GTP.
BINDING 258 258 GTP (By similarity).
MOD_RES 190 190 N6-acetyllysine (By similarity).
MOD_RES 218 218 Phosphoserine.

3D-structure; Acetylation; Cell cycle; Cell division; Cell membrane; Cell projection; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Kinetochore; Membrane; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005826; C:actomyosin contractile ring; NAS:UniProtKB.
GO:0009986; C:cell surface; IDA:MGI.
GO:0060170; C:cilium membrane; IDA:UniProtKB.
GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
GO:0000145; C:exocyst; IEA:Compara.
GO:0030496; C:midbody; TAS:MGI.
GO:0005730; C:nucleolus; IEA:Compara.
GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
GO:0031105; C:septin complex; IEA:InterPro.
GO:0005876; C:spindle microtubule; TAS:MGI.
GO:0045202; C:synapse; IDA:MGI.
GO:0030234; F:enzyme regulator activity; IDA:MGI.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; NAS:UniProtKB.
GO:0042802; F:identical protein binding; TAS:MGI.
GO:0032947; F:protein complex scaffold; IDA:MGI.
GO:0042384; P:cilium assembly; IMP:UniProtKB.
GO:0000910; P:cytokinesis; NAS:UniProtKB.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0031175; P:neuron projection development; IEA:Compara.
GO:0051258; P:protein polymerization; TAS:MGI.
GO:0002036; P:regulation of L-glutamate transport; IDA:MGI.
GO:0032880; P:regulation of protein localization; IDA:MGI.
GO:0007224; P:smoothened signaling pathway; IMP:UniProtKB.

IPR000038; Cell_div_GTP-bd.
IPR027417; P-loop_NTPase.
IPR016491; Septin.
IPR008113; Septin2.