CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010368
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase 
Protein Synonyms/Alias
 AdoMet synthase; MAT; Methionine adenosyltransferase 
Gene Name
 metK 
Gene Synonyms/Alias
 Rv1392; MT1437; MTCY21B4.09 
Created Date
 July 27, 2013 
Organism
 Mycobacterium tuberculosis 
NCBI Taxa ID
 1773 
Lysine Modification
Position
Peptide
Type
References
345TETEDPVKIEKAIGEpupylation[1]
Reference
 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 
Functional Description
 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity). 
Sequence Annotation
 NP_BIND 280 287 ATP (Potential).
 METAL 19 19 Magnesium (By similarity).
 METAL 45 45 Potassium (By similarity).
 METAL 284 284 Potassium (By similarity).
 METAL 292 292 Magnesium (By similarity).
 CROSSLNK 345 345 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 3D-structure; ATP-binding; Cobalt; Complete proteome; Cytoplasm; Isopeptide bond; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 403 AA 
Protein Sequence
MSEKGRLFTS ESVTEGHPDK ICDAISDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVT 60
TSAKEAFADI TNTVRARILE IGYDSSDKGF DGATCGVNIG IGAQSPDIAQ GVDTAHEARV 120
EGAADPLDSQ GAGDQGLMFG YAINATPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG 180
KTQVTIAYED NVPVRLDTVV ISTQHAADID LEKTLDPDIR EKVLNTVLDD LAHETLDAST 240
VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR 300
WVAKNVVAAG LAERVEVQVA YAIGKAAPVG LFVETFGTET EDPVKIEKAI GEVFDLRPGA 360
IIRDLNLLRP IYAPTAAYGH FGRTDVELPW EQLDKVDDLK RAI 403 
Gene Ontology
 GO:0005618; C:cell wall; IDA:MTBBASE.
 GO:0005829; C:cytosol; IDA:MTBBASE.
 GO:0005886; C:plasma membrane; IDA:MTBBASE.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004478; F:methionine adenosyltransferase activity; IEA:HAMAP.
 GO:0040007; P:growth; IMP:MTBBASE.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 
Pfam
 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 
SMART
  
PROSITE
 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 
PRINTS