CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031488
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAF proto-oncogene serine/threonine-protein kinase 
Protein Synonyms/Alias
 cDNA FLJ57286, highly similar to RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) 
Gene Name
 RAF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
284GSFGTVYKGKWHGDVubiquitination[1]
381GMDYLHAKNIIHRDMubiquitination[1]
389NIIHRDMKSNNIFLHubiquitination[1]
412DFGLATVKSRWSGSQubiquitination[1]
510LVADCVKKVKEERPLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 567 AA 
Protein Sequence
MMANSQILLR QATLSVFSCR TSKEQCKKAR LDWNTDAASL IGEELQVDFL DHVPLTTHNF 60
ARKTFLKLAF CDICQKFLLN GFRCQTCGYK FHEHCSTKVP TMCVDWSNIR QLLLFPNSTI 120
GDSGVPALPS LTMRRMRESV SRMPVSSQHR YSTPHAFTFN TSSPSSEGSL SQRQRSTSTP 180
NVHMVSTTLP VDSRMIEDAI RSHSESASPS ALSSSPNNLS PTGWSQPKTP VPAQRERAPV 240
SGTQEKNKIR PRGQRDSSYY WEIEASEVML STRIGSGSFG TVYKGKWHGD VAVKILKVVD 300
PTPEQFQAFR NEVAVLRKTR HVNILLFMGY MTKDNLAIVT QWCEGSSLYK HLHVQETKFQ 360
MFQLIDIARQ TAQGMDYLHA KNIIHRDMKS NNIFLHEGLT VKIGDFGLAT VKSRWSGSQQ 420
VEQPTGSVLW MAPEVIRMQD NNPFSFQSDV YSYGIVLYEL MTGELPYSHI NNRDQIIFMV 480
GRGYASPDLS KLYKNCPKAM KRLVADCVKK VKEERPLFPQ ILSSIELLQH SLPKINRSAS 540
EPSLHRAAHT EDINACTLTT SPRLPVF 567 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro. 
Interpro
 IPR020454; DAG/PE-bd.
 IPR011009; Kinase-like_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00130; C1_1
 PF07714; Pkinase_Tyr 
SMART
 SM00109; C1 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00008; DAGPEDOMAIN.