UniProt Accession

 ISPF_ECOLI; P62617; P36663; Q2MA83 

Genbank Protein ID

 L07942; AF230738; AB038256; U29579; U00096; AP009048 

Genbank Nucleotide ID

 AAA79837.1; AAF44656.1; BAA95145.1; AAA69256.1; AAC75788.1; BAE76823.1 

Protein Name

 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 

Protein Synonyms/Alias

 MECDP-synthase; MECPP-synthase; MECPS 

Gene Name

 ispF 

Gene Synonyms/Alias

 mecS; ygbB; b2746; JW2716 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
69	PDTDPAFKGADSREL	acetylation	[1]
136	VKATTTEKLGFTGRG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). Also converts 4-diphosphocytidyl-2-C-methyl-D-erythritol into 2-C- methyl-D-erythritol 3,4-cyclophosphate and CMP. 

Sequence Annotation

 REGION 8 10 Substrate binding.
 REGION 34 35 Substrate binding.
 REGION 38 46 Substrate binding.
 REGION 56 58 Substrate binding.
 REGION 61 65 Substrate binding.
 REGION 100 106 Substrate binding.
 REGION 131 135 Substrate binding.
 METAL 8 8 Divalent metal cation.
 METAL 10 10 Divalent metal cation.
 METAL 42 42 Divalent metal cation.
 BINDING 65 65 Substrate; via carbonyl oxygen.
 BINDING 139 139 Substrate; via carbonyl oxygen.
 BINDING 142 142 Substrate.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Isoprene biosynthesis; Lyase; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 159 AA 

Protein Sequence

Gene Ontology

 GO:0008685; F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
 GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
 GO:0006744; P:ubiquinone biosynthetic process; IDA:EcoCyc. 

Interpro

 IPR003526; MECDP_synthase.
 IPR020555; MECDP_synthase_CS. 

Pfam

 PF02542; YgbB 

SMART

  

PROSITE

 PS01350; ISPF 

PRINTS