CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021440
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative ATP-dependent RNA helicase DHX33 
Protein Synonyms/Alias
 DEAH box protein 33 
Gene Name
 DHX33 
Gene Synonyms/Alias
 DDX33 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103IGETGSGKTTQIPQYubiquitination[1, 2]
168TSEDTRIKFLTDGMLubiquitination[3]
187ISDSLLRKYSCVILDubiquitination[3]
313KTCRDIAKHLPDGCPubiquitination[3]
345RVFQGAPKGYRKVIIubiquitination[3]
376VVDTGMVKAKKYNPDubiquitination[1, 3]
378DTGMVKAKKYNPDSGubiquitination[3]
379TGMVKAKKYNPDSGLubiquitination[3]
396LAVQRVSKTQAWQRTubiquitination[3]
425YTEDEFEKFDKMTVPubiquitination[3]
428DEFEKFDKMTVPEIQubiquitination[3]
484LLGALEHKDDQLTLTubiquitination[1]
580GGNKDWCKENFVNSKubiquitination[3]
587KENFVNSKNMTLVAEubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization. 
Sequence Annotation
 DOMAIN 84 252 Helicase ATP-binding.
 DOMAIN 277 450 Helicase C-terminal.
 NP_BIND 97 104 ATP (By similarity).
 MOTIF 194 197 DEAH box.
 MOTIF 547 558 Critical for rDNA-binding (By
 MOD_RES 134 134 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 707 AA 
Protein Sequence
MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR QQPPLAQPSA 60
SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG SGKTTQIPQY LYEGGISRQG 120
IIAVTQPRRV AAISLATRVS DEKRTELGKL VGYTVRFDDV TSEDTRIKFL TDGMLLREAI 180
SDSLLRKYSC VILDEAHERT IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS 240
QYFNGAPVLY LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE 300
IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV IISTNIAETS 360
ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW QRTGRAGRED SGICYRLYTE 420
DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA 480
LEHKDDQLTL TPMGRKMAAF PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS 540
RREEVQGVRK KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR 600
DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV AIHPSSVLFH 660
CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR KLRTARN 707 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0000182; F:rDNA binding; IDA:UniProtKB.
 GO:0045943; P:positive regulation of transcription from RNA polymerase I promoter; IDA:UniProtKB. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS