CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016255
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Testis-specific Y-encoded-like protein 5 
Protein Synonyms/Alias
 TSPY-like protein 5 
Gene Name
 TSPYL5 
Gene Synonyms/Alias
 KIAA1750 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108AARPGPGKAASLSERubiquitination[1]
133AGTVGRPKNAPRVGNubiquitination[1, 2]
307RNPYFQNKVLIKEYGacetylation[3]
307RNPYFQNKVLIKEYGubiquitination[1, 2]
311FQNKVLIKEYGCGPSubiquitination[1, 2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53 protein levels and promotes its ubiquitination; the function is dependent on USP7 and independent on MDM2. Proposed to displace p53/TP53 from interaction with USP7. 
Sequence Annotation
  
Keyword
 Complete proteome; Growth regulation; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 417 AA 
Protein Sequence
MSGRSRGRKS SRAKNRGKGR AKARVRPAPD DAPRDPDPSQ YQSLGEDTQA AQVQAGAGWG 60
GLEAAASAQL LRLGEEAACR LPLDCGLALR ARAAGDHGQA AARPGPGKAA SLSERLAADT 120
VFVGTAGTVG RPKNAPRVGN RRGPAGKKAP ETCSTAGRGP QVIAGGRQKK GAAGENTSVS 180
AGEEKKEERD AGSGPPATEG SMDTLENVQL KLENMNAQAD RAYLRLSRKF GQLRLQHLER 240
RNHLIQNIPG FWGQAFQNHP QLASFLNSQE KEVLSYLNSL EVEELGLARL GYKIKFYFDR 300
NPYFQNKVLI KEYGCGPSGQ VVSRSTPIQW LPGHDLQSLS QGNPENNRSF FGWFSNHSSI 360
ESDKIVEIIN EELWPNPLQF YLLSEGARVE KGKEKEGRQG PGKQPMETTQ PGVSQSN 417 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0071480; P:cellular response to gamma radiation; IMP:UniProtKB.
 GO:0006334; P:nucleosome assembly; IEA:InterPro.
 GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IMP:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW. 
Interpro
 IPR002164; NAP_family. 
Pfam
 PF00956; NAP 
SMART
  
PROSITE
  
PRINTS