UniProt Accession

 LGMN_HUMAN; Q99538; O00123; Q9BTY1 

Genbank Protein ID

 Y09862; D55696; BC003061 

Genbank Nucleotide ID

 CAA70989.1; BAA09530.1; AAH03061.1 

Protein Name

 Legumain 

Protein Synonyms/Alias

 Asparaginyl endopeptidase; Protease, cysteine 1 

Gene Name

 LGMN 

Gene Synonyms/Alias

 PRSC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
125	RGDAEAVKGIGSGKV	ubiquitination	[1, 2]
131	VKGIGSGKVLKSGPQ	ubiquitination	[2]
224	YACYYDEKRSTYLGD	ubiquitination	[2]
316	DVPLTIMKRKLMNTN	ubiquitination	[1, 2]
318	PLTIMKRKLMNTNDL	ubiquitination	[1]
347	DARHLIEKSVRKIVS	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. 

Sequence Annotation

 ACT_SITE 148 148 Potential.
 ACT_SITE 189 189 Potential.
 CARBOHYD 91 91 N-linked (GlcNAc...).
 CARBOHYD 167 167 N-linked (GlcNAc...).
 CARBOHYD 263 263 N-linked (GlcNAc...) (Potential).
 CARBOHYD 272 272 N-linked (GlcNAc...) (Potential).  

Keyword

 Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Lysosome; Polymorphism; Protease; Reference proteome; Signal; Thiol protease; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 433 AA 

Protein Sequence

Gene Ontology

 GO:0045177; C:apical part of cell; IEA:Compara.
 GO:0005770; C:late endosome; IEA:Compara.
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0040015; P:negative regulation of multicellular organism growth; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IGI:MGI.
 GO:0006508; P:proteolysis; ISS:UniProtKB.
 GO:0001101; P:response to acid; IEA:Compara.
 GO:0002224; P:toll-like receptor signaling pathway; TAS:Reactome.
 GO:0042359; P:vitamin D metabolic process; TAS:Reactome. 

Interpro

 IPR001096; Peptidase_C13. 

Pfam

 PF01650; Peptidase_C13 

SMART

  

PROSITE

  

PRINTS

 PR00776; HEMOGLOBNASE.