CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011118
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA (guanine-N(7)-)-methyltransferase subunit TRM82 
Protein Synonyms/Alias
 Transfer RNA methyltransferase 82 
Gene Name
 TRM82 
Gene Synonyms/Alias
 YDR165W; YD8358.19 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
31KNCILSFKYQSPNHWubiquitination[1]
90SIKRTAAKVPSPGLGacetylation[2]
385GVQKNFAKFIEYNLNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit. 
Sequence Annotation
 REPEAT 1 47 WD 1.
 REPEAT 48 99 WD 2.
 REPEAT 100 147 WD 3.
 REPEAT 148 192 WD 4.
 REPEAT 193 237 WD 5.
 REPEAT 238 279 WD 6.
 REPEAT 308 354 WD 7.
 MOD_RES 93 93 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; tRNA processing; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 444 AA 
Protein Sequence
MSVIHPLQNL LTSRDGSLVF AIIKNCILSF KYQSPNHWEF AGKWSDDFDK IQESRNTTAK 60
EQQGQSSENE NENKKLKSNK GDSIKRTAAK VPSPGLGAPP IYSYIRNLRL TSDESRLIAC 120
ADSDKSLLVF DVDKTSKNVL KLRKRFCFSK RPNAISIAED DTTVIIADKF GDVYSIDINS 180
IPEEKFTQEP ILGHVSMLTD VHLIKDSDGH QFIITSDRDE HIKISHYPQC FIVDKWLFGH 240
KHFVSSICCG KDYLLLSAGG DDKIFAWDWK TGKNLSTFDY NSLIKPYLND QHLAPPRFQN 300
ENNDIIEFAV SKIIKSKNLP FVAFFVEATK CIIILEMSEK QKGDLALKQI ITFPYNVISL 360
SAHNDEFQVT LDNKESSGVQ KNFAKFIEYN LNENSFVVNN EKSNEFDSAI IQSVQGDSNL 420
VTKKEEIYPL YNVSSLRKHG EHYS 444 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0043527; C:tRNA methyltransferase complex; IDA:SGD.
 GO:0036265; P:RNA (guanine-N7)-methylation; IDA:GOC.
 GO:0030488; P:tRNA methylation; IDA:SGD. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS