CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008345
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium/potassium-transporting ATPase subunit alpha-2 
Protein Synonyms/Alias
 Na(+)/K(+) ATPase alpha-2 subunit; Sodium pump subunit alpha-2 
Gene Name
 ATP1A2 
Gene Synonyms/Alias
 KIAA0778 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43EVAMDDHKLSLDELGubiquitination[1]
375TSTICSDKTGTLTQNubiquitination[1]
602AVPDAVGKCRSAGIKubiquitination[1, 2]
609KCRSAGIKVIMVTGDubiquitination[1]
622GDHPITAKAIAKGVGubiquitination[1]
626ITAKAIAKGVGIISEubiquitination[1]
695ARTSPQQKLIIVEGCubiquitination[2]
723VNDSPALKKADIGIAubiquitination[1]
770RLIFDNLKKSIAYTLubiquitination[1]
947SVFQQGMKNKILIFGubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. 
Sequence Annotation
 REGION 80 82 Interaction with phosphoinositide-3
 ACT_SITE 374 374 4-aspartylphosphate intermediate (By
 METAL 714 714 Magnesium (By similarity).
 METAL 718 718 Magnesium (By similarity).
 MOD_RES 570 570 Phosphothreonine.
 MOD_RES 587 587 Phosphoserine.
 MOD_RES 650 650 Phosphoserine (By similarity).
 MOD_RES 940 940 Phosphoserine; by PKA (By similarity).  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Disease mutation; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Sodium; Sodium transport; Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1020 AA 
Protein Sequence
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG RKYQVDLSKG 60
LTNQRAQDVL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI LLWIGAILCF LAYGIQAAME 120
DEPSNDNLYL GVVLAAVVIV TGCFSYYQEA KSSKIMDSFK NMVPQQALVI REGEKMQINA 180
EEVVVGDLVE VKGGDRVPAD LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI 240
CFFSTNCVEG TARGIVIATG DRTVMGRIAT LASGLEVGRT PIAMEIEHFI QLITGVAVFL 300
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA RKNCLVKNLE 360
AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT TEDQSGATFD KRSPTWTALS 420
RIAGLCNRAV FKAGQENISV SKRDTAGDAS ESALLKCIEL SCGSVRKMRD RNPKVAEIPF 480
NSTNKYQLSI HEREDSPQSH VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM 540
ELGGLGERVL GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV 600
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPMS QVNPREAKAC 660
VVHGSDLKDM TSEQLDEILK NHTEIVFART SPQQKLIIVE GCQRQGAIVA VTGDGVNDSP 720
ALKKADIGIA MGISGSDVSK QAADMILLDD NFASIVTGVE EGRLIFDNLK KSIAYTLTSN 780
IPEITPFLLF IIANIPLPLG TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK 840
LVNERLISMA YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TMNDLEDSYG 900
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI LIFGLLEETA 960
LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE VRKLILRRYP GGWVEKETYY 1020 
Gene Ontology
 GO:0005901; C:caveola; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0043197; C:dendritic spine; IEA:Compara.
 GO:0005768; C:endosome; IEA:Compara.
 GO:0005890; C:sodium:potassium-exchanging ATPase complex; IC:UniProtKB.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0030315; C:T-tubule; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005391; F:sodium:potassium-exchanging ATPase activity; IMP:UniProtKB.
 GO:0008344; P:adult locomotory behavior; IEA:Compara.
 GO:0006754; P:ATP biosynthetic process; IEA:InterPro.
 GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:Compara.
 GO:0071260; P:cellular response to mechanical stimulus; IEA:Compara.
 GO:0040011; P:locomotion; IEA:Compara.
 GO:0045822; P:negative regulation of heart contraction; IEA:Compara.
 GO:0045988; P:negative regulation of striated muscle contraction; IEA:Compara.
 GO:0001504; P:neurotransmitter uptake; IEA:Compara.
 GO:0051481; P:reduction of cytosolic calcium ion concentration; IEA:Compara.
 GO:0008217; P:regulation of blood pressure; IEA:Compara.
 GO:0086004; P:regulation of cardiac muscle cell contraction; IEA:Compara.
 GO:0002087; P:regulation of respiratory gaseous exchange by neurological system process; IEA:Compara.
 GO:0006940; P:regulation of smooth muscle contraction; IEA:Compara.
 GO:0006942; P:regulation of striated muscle contraction; NAS:UniProtKB.
 GO:0002026; P:regulation of the force of heart contraction; IEA:Compara.
 GO:0019229; P:regulation of vasoconstriction; IEA:Compara.
 GO:0035094; P:response to nicotine; IEA:Compara.
 GO:0008542; P:visual learning; IEA:Compara. 
Interpro
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR005775; ATPase_P-typ_Na/K_IIC.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.