UniProt Accession

 NUCG_HUMAN; Q14249; Q5T281; Q9BSP2 

Genbank Protein ID

 X79444; AL441992; BC004922; BC016351 

Genbank Nucleotide ID

 CAA55963.1; CAI15412.1; AAH04922.1; AAH16351.1 

Protein Name

 Endonuclease G, mitochondrial 

Protein Synonyms/Alias

 Endo G 

Gene Name

 ENDOG 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
66	RGPGELAKYGLPGLA	ubiquitination	[1, 2]
76	LPGLAQLKSRESYVL	ubiquitination	[1, 2, 3, 4, 5]
153	ANHRWSQKAMDDTFY	ubiquitination	[1, 2, 3, 4]
181	NAWNNLEKYSRSLTR	ubiquitination	[1, 2]
210	PRTEADGKSYVKYQV	ubiquitination	[1, 2]
214	ADGKSYVKYQVIGKN	ubiquitination	[1, 2, 3, 4, 5, 6]
220	VKYQVIGKNHVAVPT	ubiquitination	[2, 6]
290	LARAGSLKAITAGSK	ubiquitination	[1, 2, 3, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity). 

Sequence Annotation

 ACT_SITE 141 141 Proton acceptor (By similarity).
 METAL 172 172 Magnesium or manganese; catalytic (By  

Keyword

 Complete proteome; Endonuclease; Hydrolase; Magnesium; Manganese; Metal-binding; Mitochondrion; Nuclease; Polymorphism; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006309; P:apoptotic DNA fragmentation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0046677; P:response to antibiotic; IEA:Compara.
 GO:0034612; P:response to tumor necrosis factor; IEA:Compara. 

Interpro

 IPR018524; DNA/RNA_endonuclease_AS.
 IPR001604; DNA/RNA_non-sp_Endonuclease.
 IPR020821; Extracellular_endonuc_su_A. 

Pfam

 PF01223; Endonuclease_NS 

SMART

 SM00892; Endonuclease_NS
 SM00477; NUC 

PROSITE

 PS01070; NUCLEASE_NON_SPEC 

PRINTS