CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013712
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine--tRNA ligase 
Protein Synonyms/Alias
 Tyrosyl-tRNA synthetase; TyrRS 
Gene Name
 tyrS 
Gene Synonyms/Alias
 GK2803 
Created Date
 July 27, 2013 
Organism
 Geobacillus kaustophilus (strain HTA426) 
NCBI Taxa ID
 235909 
Lysine Modification
Position
Peptide
Type
References
91SERTLNAKETVEAWSacetylation[1]
Reference
 [1] Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus.
 Lee DW, Kim D, Lee YJ, Kim JA, Choi JY, Kang S, Pan JG.
 Proteomics. 2013 Aug;13(15):2278-82. [PMID: 23696451] 
Functional Description
 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 
Sequence Annotation
 DOMAIN 352 419 S4 RNA-binding.
 MOTIF 39 48 "HIGH" region.
 MOTIF 230 234 "KMSKS" region.
 BINDING 34 34 Tyrosine (By similarity).
 BINDING 169 169 Tyrosine (By similarity).
 BINDING 173 173 Tyrosine (By similarity).
 BINDING 233 233 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 419 AA 
Protein Sequence
MDLLAELQWR GLVNQTTDED GLRKLLNEER VTLYCGFDPT ADSLHIGNLA AILTLRRFQQ 60
AGHRPIALVG GATGLIGDPS GKKSERTLNA KETVEAWSAR IKEQLGRFLD FEADGNPAKI 120
KNNYDWIGPL DVITFLRDVG KHFSVNYMMA KESVQSRIET GISFTEFSYM MLQAYDFLRL 180
YETEGCRLQI GGSDQWGNIT AGLELIRKTK GEARAFGLTI PLVTKADGTK FGKTESGTIW 240
LDKEKTSPYE FYQFWINTDD RDVIRYLKYF TFLSKEEIEA LEQELREAPE KRAAQKALAE 300
EVTKLVHGEE ALRQAIRISE ALFSGDIANL TAAEIEQGFK DVPSFVHEGG DVPLVELLVS 360
AGISPSKRQA REDIQSGAIY VNGERLQDVG AILTAEHRLE GRFTVIRRGK KKYYLIRYA 419 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:HAMAP.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type.
 IPR024107; Tyr-tRNA-ligase_bac_1. 
Pfam
 PF01479; S4
 PF00579; tRNA-synt_1b 
SMART
 SM00363; S4 
PROSITE
 PS00178; AA_TRNA_LIGASE_I
 PS50889; S4 
PRINTS
 PR01040; TRNASYNTHTYR.