CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002325
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2-isopropylmalate synthase 
Protein Synonyms/Alias
 Alpha-IPM synthase; Alpha-isopropylmalate synthase 
Gene Name
 LEU4 
Gene Synonyms/Alias
 YNL104C; N2173 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
25SRVIPPVKLAYKNMLacetylation[1]
33LAYKNMLKDPSSKYKacetylation[1]
40KDPSSKYKPFNAPKLacetylation[1]
90QKKEYFHKLVNIGFKacetylation[1]
178SREEAISKAVEATKLacetylation[1]
184SKAVEATKLVRKLTKacetylation[1]
374GSHQDAIKKGFNLQNacetylation[1]
401PYLPLDPKDIGRDYEacetylation[1]
464LKSDEISKLFKEAYNacetylation[1]
567RRNADNEKAYKWGVGacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). 
Sequence Annotation
  
Keyword
 Alternative initiation; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Mitochondrion; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 619 AA 
Protein Sequence
MVKESIIALA EHAASRASRV IPPVKLAYKN MLKDPSSKYK PFNAPKLSNR KWPDNRITRA 60
PRWLSTDLRD GNQSLPDPMS VEQKKEYFHK LVNIGFKEIE VSFPSASQTD FDFTRYAVEN 120
APDDVSIQCL VQSREHLIKR TVEALTGAKK ATIHTYLATS DMFREIVFNM SREEAISKAV 180
EATKLVRKLT KDDPSQQATR WSYEFSPECF SDTPGEFAVE ICEAVKKAWE PTEENPIIFN 240
LPATVEVASP NVYADQIEYF ATHITEREKV CISTHCHNDR GCGVAATELG MLAGADRVEG 300
CLFGNGERTG NVDLVTVAMN MYTQGVSPNL DFSDLTSVLD VVERCNKIPV SQRAPYGGDL 360
VVCAFSGSHQ DAIKKGFNLQ NKKRAQGETQ WRIPYLPLDP KDIGRDYEAV IRVNSQSGKG 420
GAAWVILRSL GLDLPRNMQI EFSSAVQDHA DSLGRELKSD EISKLFKEAY NYNDEQYQAI 480
SLVNYNVEKF GTERRVFTGQ VKVGDQIVDI EGTGNGPISS LVDALSNLLN VRFAVANYTE 540
HSLGSGSSTQ AASYIHLSYR RNADNEKAYK WGVGVSEDVG DSSVRAIFAT INNIIHSGDV 600
SIPSLAEVEG KNAAASGSA 619 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0003852; F:2-isopropylmalate synthase activity; IDA:SGD.
 GO:0009098; P:leucine biosynthetic process; IGI:SGD. 
Interpro
 IPR013709; 2-isopropylmalate_synth_dimer.
 IPR002034; AIPM/Hcit_synth_CS.
 IPR013785; Aldolase_TIM.
 IPR005668; IPM_Synthase.
 IPR000891; PYR_CT. 
Pfam
 PF00682; HMGL-like
 PF08502; LeuA_dimer 
SMART
 SM00917; LeuA_dimer 
PROSITE
 PS00815; AIPM_HOMOCIT_SYNTH_1
 PS00816; AIPM_HOMOCIT_SYNTH_2
 PS50991; PYR_CT 
PRINTS