CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007900
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar protein 9 
Protein Synonyms/Alias
 Pumilio domain-containing protein NOP9 
Gene Name
 NOP9 
Gene Synonyms/Alias
 YJL010C; J1357 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
238KKSKIARKMIDIKDNacetylation[1]
637FEIFPNSKPLQPKPEacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus. 
Sequence Annotation
 DOMAIN 23 435 PUM-HD.
 REPEAT 92 127 Pumilio 1.
 REPEAT 128 163 Pumilio 2.
 REPEAT 188 223 Pumilio 3.
 REPEAT 286 326 Pumilio 4.
 REPEAT 334 368 Pumilio 5.
 REPEAT 369 407 Pumilio 6.
 REPEAT 511 548 Pumilio 7.
 REPEAT 549 587 Pumilio 8.  
Keyword
 Complete proteome; Nucleus; Reference proteome; Repeat; Ribosome biogenesis; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 666 AA 
Protein Sequence
MGKTKTRGRR HQDKQRKDEF EPSSNSAKEH IQQEESTYND EAEIKETQPQ MFFGVLDREE 60
LEYFKQAEST LQLDAFEAPE EKFQFVTSII EEAKGKELKL VTSQITSKLM ERVILECDET 120
QLKDIFQSFN GVFFGLSCHK YASHVLETLF VRSAALVERE LLTPSFDNNE KEGPYVTMEN 180
MFLFMLNELK PHLKTMMNHQ YASHVLRLLI LILSSKTLPN STKANSTLRS KKSKIARKMI 240
DIKDNDDFNK VYQTPESFKS ELRDIITTLY KGFTNGAESR SDISQSTITK FREYSVDKVA 300
SPVIQLIIQV EGIFDRDRSF WRLVFNTADE KDPKEESFLE YLLSDPVGSH FLENVIGSAR 360
LKYVERLYRL YMKDRIVKLA KRDTTGAFVV RALLEHLKEK DVKQILDAVV PELSMLLNSN 420
MDFGTAIINA SNKQGGYLRD DVIAQLIQKY YPEKSDAKNI LESCLLLSAS TLGNTRDDWP 480
TAEERRRSVF LEQLIDYDDK FLNITIDSML ALPEERLIQM CYHGVFSHVV EHVLQTTRVD 540
IIKRKMLLNI LSKESVNLAC NVYGSHIMDK LWEFTAKLTL YKERIARALV LETEKVKNSI 600
YGRQVWKNWK LELYVRKMWD WKKLIKEQEF EIFPNSKPLQ PKPEKHSRER NNSKEGSAFK 660
KQKHYR 666 
Gene Ontology
 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0030688; C:preribosome, small subunit precursor; IDA:SGD.
 GO:0003723; F:RNA binding; IDA:SGD.
 GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001313; Pumilio_RNA-bd_rpt. 
Pfam
 PF00806; PUF 
SMART
 SM00025; Pumilio 
PROSITE
 PS50302; PUM
 PS50303; PUM_HD 
PRINTS